超微粉碎萃取技术与湿法粉碎萃取技术在棉籽蛋白加工中的应用探索

Application of ultrafine pulverization extraction technology and wet pulverization extraction technology in cottonseed protein processing

对超微粉碎萃取技术和湿法粉碎萃取技术在棉籽蛋白加工中的应用进行探索。以棉籽蛋白残油量及棉酚含量为考察指标,确定棉籽蛋白超微粉碎萃取、湿法粉碎萃取最佳工艺条件。结果表明,棉籽蛋白超微粉碎萃取较佳条件为:物料粒度120～180μm,单次萃取时间5 min,正己烷萃取4次,甲醇-乙醇(2∶1)萃取3次,总有效萃取时间35 min;在较佳条件下,得到残油量为0. 29%、棉酚含量为648 mg/kg的棉籽蛋白。棉籽蛋白湿法粉碎萃取较佳条件为:棉籽仁与正己烷湿法多级粉碎5次,甲醇-乙醇(2∶1)萃取3次,单次萃取时间0. 5 min,总有效萃取时间4 min;在较佳条件下,得到残油量为0. 9%、棉酚含量为1 841 mg/kg的棉籽蛋白。

The application of ultrafine pulverization extraction technology and wet pulverization extraction technology in cottonseed protein processing were studied. The process conditions of ultrafine pulverization extraction and wet pulverization extraction were optimized with contents of residual oil and gossypol as indicators. The results showed that the optimal conditions of ultrafine pulverization extraction were obtained as follows: material particle size 120-180 μm,single extraction time 5 min,extraction times4 with n-hexane for defatting,extraction times 3 with methanol-ethanol( 2∶ 1) for removing gossypol,and total effective extraction time 35 min. Under these conditions,the contents of residual oil and gossypol in cottonseed protein were 0. 29% and 648 mg/kg. The optimal conditions for wet pulverization extraction were obtained as follows: cottonseed kernel and n-hexane wet multi-stage crushing times 5,extraction times 3 with methanol-ethanol( 2∶ 1),single extraction time 0. 5 min and total effective extraction time 4 min. Under these conditions,the contents of residual oil and gossypol in cottonseed protein were 0. 9% and 1 841 mg/kg.