纤维素酶结合碱性蛋白酶提高冷榨大豆出油率的工艺优化

Optimization of process of cellulase and alkaline protease for improving soybean oil yield by cold-pressing

以大豆为原料,采用冷榨技术制取大豆油,以出油率为评价指标,通过单因素试验和正交试验优化纤维素酶与碱性蛋白酶结合应用提高冷榨大豆出油率工艺条件。结果表明:复合酶冷榨大豆油的最佳工艺参数为物料粒径40目、水分含量13%、冷榨温度80℃、螺杆转速36 r/min、复合酶(纤维素酶液与碱性蛋白酶液体积比1∶2)用量0. 2%,在此条件下大豆出油率为70. 6%,优于未经酶处理的常规冷榨工艺的大豆出油率(68. 5%),出油率提高了2. 1个百分点。所得大豆油具有大豆油特有的气味和滋味,呈棕红色,且澄清、无杂质,酸价(KOH)为0. 8 mg/g、过氧化值为6. 1 mmol/kg。

Soybean oil was prepared by cold-pressing technology with the oil yield as evaluation index.The process conditions of the combination of cellulase and alkaline protease for improving the cold-pressed soybean oil yield were optimized by single factor experiment and orthogonal experiment.The compound enzymatic cold-pressing conditions were determined as follows:particle size 40 meshes,moisture content 13%,cold-pressing temperature 80℃,screw speed 36 r/min and compound enzyme addition0.2%(volume ratio of cellulase to alkaline protease 1∶2).Under these conditions,the oil yield was70.6%,significantly higher by 2.1 percentage points than 68.5%of the cold-pressing process without enzyme treatment.The obtained soybean oil had unique aroma and flavor of soybean oil with brownish red,which was clear without impurities,and the acid value and peroxide value were 0.8 mgKOH/g and 6.1 mmol/kg respectively.