响应面法优化辣木籽油超声辅助提取工艺及其脂肪酸组成

Optimization of ultrasound-assisted extraction process of Moringa oleifera seed oil by response surface methodology and its fatty acid composition

以正己烷为提取溶剂,选取超声功率、提取温度、提取时间、料液比为影响因素进行单因素试验,在此基础上利用响应面法对超声辅助正己烷提取辣木籽油的工艺条件进行了优化,并对所得辣木籽油的脂肪酸组成进行了分析。结果表明:辣木籽油的最佳提取工艺条件为料液比1∶9、提取时间39 min、提取温度35℃、超声功率120 W,在此条件下辣木籽出油率为36. 10%,提取效率达96. 52%;辣木籽油经气相色谱-质谱联用技术分析共鉴定出21种脂肪酸,其中不饱和脂肪酸11种(含量80. 10%),饱和脂肪酸10种(含量19. 89%),且单不饱和脂肪酸油酸含量最高,为65. 94%,饱和脂肪酸棕榈酸含量次之,为7. 21%。

The process for ultrasound-assisted n-hexane extraction of Moringa oleifera seed zoil was optimized by response surface methodology based on single factor experiment,and the fatty acid composition of the Moringa oleifera seed oil was analyzed by GC-MS.The results showed that the optimal extraction condtions of Moringa oleifera seed oil were obtained as follows:ratio of material to liquid 1∶9,extraction time 39 min,extraction temperature 35℃,ultrasonic power 120 W.Under the optimal conditions,the Moringa oleifera seed oil yield was 36.10%and the extraction efficiency was 96.52%.A total of 21 fatty acids were identified from the extracted Moringa oleifera seed oil by GC-MS,among which eleven compounds were unsaturated fatty acids and saturated fatty acids covered ten compounds.The oleic acid content was the highest,accounting for 65.94%,and the second was palmitic acid,accounting for 7.21%.