QuEChERS/气相色谱三重四级杆质谱法测定枸杞籽油中多环芳烃类物质

Determination of PAHs in Lycium seed oil by Qu ECh ERS/GC-MS/MS

建立了同时检测保健食品枸杞籽油中16种多环芳烃(PAHs)的QuEChERS/气相色谱三重四级杆质谱(GC-MS/MS)方法。样品(0. 5 g)经3 mL乙腈提取2次,采用含200 mg PSA和100 mg C18吸附填料粉进行净化,净化后采用GC-MS/MS进行分析测定。结果表明:在优化条件下,16种PAHs分离度良好,在1. 0~200 ng/mL范围内线性关系良好;回收率为60. 04%~119. 00%,RSD在2. 92%~13. 03%之间;检出限在0. 2~3. 5μg/kg之间,定量限在0. 7~11. 5μg/kg之间。该方法数据可靠、简便快速、灵敏度高,适合保健食品枸杞籽油中多环芳烃的测定。

A QuEChERS with gas chromatography-triple quadrupole mass spectrometry(GC-MS/MS)method was developed for the simultaneous determination of 16 PAHs in Lycium seed oil.The PAHs compounds were isolated fromLycium seed oil(0.5 g)by extracting twice with 3 mL acetonitrile,then purified with 200 mg PSA and 100 mg C18 packing powder.The purified liquid containing 16 PAHs were analyzed by GC-MS/MS.Under the optimal conditions,the separability of 16 PAHs was good,and the method had good linear relationship in the range of 1.0-200 ng/mL.The recovery rate was 60.04%-119.00%with RSD 2.92%-13.03%.The detection limit and quantitative limit were in the rangs of0.2-3.5μg/kg and 0.7-11.5μg/kg.The method had advantages of reliable data,simplicity,rapidness and high sensitivity,and it was suitable for the determination of PAHs in Lycium seed oil.