川芎嗪对大鼠加速型抗GBM抗体肾炎肾皮质的线粒体膜流动性和ATP酶活性的影响

Effects of tetramethylpyrazine on membrane fluidity and ATPase activity of renal cortex mitochondria in accelerated anti-GBM antibody nephritis of rats

目的 观察川芎嗪对大鼠加速型抗肾小球基底膜 (GBM)抗体肾炎肾皮质线粒体膜流动性和ATP酶活性的影响。方法 采用 1,6 -二苯基 - 1,3,5 -己三烯 (DPH)荧光偏振法研究抗GBM肾炎肾皮质的线粒体膜流动性 ,利用定磷法测定线粒体膜ATP酶的活性。结果 川芎嗪能调节抗GBM抗体肾炎肾皮质的线粒体膜流动性 ,同时可以使Na+ -K+ -ATPase、Mg2 + -ATPase、Ca2 + -ATPase活力升高。结论 川芎嗪可能通过改善肾线粒体膜流动性 ,而保护线粒体膜结构及功能。

OBJECTIVE To investigate the effects of tetramethylpyrazine(TMP) on membrane fluidity and ATPase activity of renal cortex mitochondria in accelerated anti-glomerulus basement membrane(GBM) antibody nephritis of rats.METHODS The changes in membrane fluidity of renal cortex mitochondria in accelerated anti-GBM antibody nephritis of rats were measured by 1,6-diphenyl-1,3,5-hexatriene(DPH).The activity of ATPase was detected by determining phosphorum.RESULTS TMP could increase membrane fluidity and the activity of Na +-K +-ATPase,Mg 2+-ATPase and Ca 2+-ATPase.CONCLUSION TMP can protect mitochondria structure and function through improving membrane fluidity.