川芎嗪对大鼠加速型抗GBM肾炎血清抗体补体的影响

Effects of tetramethylpyrazine on serum antibody and complements of accelerated anti-GBM nephritis in rats

目的 探讨川芎嗪 (TMP)对大鼠加速型抗肾小球基底膜 (GBM)肾炎血清抗体补体水平的影响。方法 建立大鼠加速型抗GBM肾炎模型 ,给药组于造模前一天ipTMP 12 0mg·kg-1·d-1,连续 15d ,于造模后第 1、3、5、7、10、14天测 2 4h尿蛋白含量 ;并于第 7、14天分两批处死大鼠 ,测定血清尿素氮 (BUN)、肌酐 (Scr)含量及IgG、IgM、IgA、C3 水平的变化。结果 给药组与模型组比较 ,尿蛋白含量显著降低 (P <0 .0 1) ;14dBUN、Scr的含量显著减少 (P <0 .0 1) ;第 7、14天血清IgG和第 14天C3 水平明显升高 (P <0 .0 1) ,且随给药时间延长而升高 ;IgM随给药时间逐渐降低 ,到 14d时更为明显 (P <0 .0 5 ) ;IgA在给药前后无明显变化。结论 TMP对大鼠加速型抗GBM肾炎有保护作用 ,其可能机制是干预了C3 、IgG、IgM参与疾病的发生发展过程。

OBJECTIVE To investigate the effects of tetramethylpyrazine(TMP) on serum antibody and complements in accelerated anti-glomerulus basement membrane(GBM) nephritis of rats.METHODS Accelerated anti-GBM nephritis model was established with rabbit anti-rat GBM antibody injection in rats.TMP was administrated 120 mg·kg -1·d -1 by ip the day before the model established and the administration was consecutive for 15 days.Content of urine protein in 24 hours was detected after model established and rats were killed in 7th day and 14th day.Contents of blood urine nitrogen (BUN) and serum creatinine (Scr) were detected.And the changes of IgG、IgM、IgA、C 3 in serum were determined.RESULTS Compared with model group,TMP group was found to have significant inhibition in changes of proteinuria.And BUN,Scr decreased significantly(P<0.01) after 14 days.The concentration of IgG increased significantly in 7th,14th day and C 3 increased in 14th day significantly(P<0.01).All the indexes increased with administration time going on,while the concentration of IgM decreased,it was more obvious in 14th day.The concentration of IgA changed little before and after administration.CONCLUSION TMP can bring protective effects on rats of accelerated anti-GBM nephritis and its probable mechanism is to interfere C 3,IgG,IgM in the process of disease generation and development.