饲料构成影响大鼠不同组织中UCP2 mRNA的表达

Effects of different diet composition on the expression of UCP2 mRNA in different tissues of rat

目的 探讨不同饲料构成对大鼠褐色脂肪组织、白色脂肪组织与骨骼肌解偶联蛋白 - 2基因表达的影响。方法 利用不同的饲料 (包括基础饲料、高蛋白、高脂肪饲料、高能饲料 1与高能饲料 2 )喂养雄性Wistar大鼠 2个月 ,观察不同饲料构成对大鼠体重、脂体比与游离脂肪酸的影响 ,并应用RT PCR方法则定大鼠褐色脂肪组织、白色脂肪组织与骨骼肌UCP2mRNA表达水平。结果 高能饲料 1组与高能饲料 2组的体重、脂体比与游离脂肪酸均显著高于基础组、高蛋白组与高脂肪组 (P <0 0 5 ) ,而后三者的体重、脂体比与游离脂肪酸无显著性差异 (P >0 0 5 )。高能饲料 1与高能饲料 2组白色脂肪组织UCP2基因表达增强 ,而能量相同的基础组、高蛋白组与高脂肪组没有明显差异。各组褐色脂肪组织与骨骼肌UCP2mRNA水平相近。结论 能量能诱导大鼠白色脂肪组织UCP2mRNA表达 ,表达的强弱与体内的储存能量多少有关。褐色脂肪组织与骨骼肌UCP2mRAN表达不受饲料成分的影响。

Objective In order to explore effect of different diet composition on uncoupling proteins 2(UCP2) gene expression in rat brown adipose tissue, white adipose tissue and skeletal muscle. Methods Male Wistar rats were fed with different diets (including basic diet, high protein, high fat, high calorie 1 and 2 diet) for 2 months. The effects of the different diets on weight, the ratio of fat to body weight and free fatty acids were observed. Reverse transcription-polymerase chain reaction (RT-PCR) technique was used to measure the expression of UCP2 mRNA in rat brown adipose tissue, white adipose tissue and skeletal muscle. Results Body weight, the ratio of rat to body weight and free fatty acids in high calorie 1 and 2 diet groups are obviously higher than that in groups of basic diet, high protein, high fat diet (P<0.05), but there is no significant difference between the last three groups (P>0.05). UCP2 gene expression in white adipose tissue increases in high calorie diet 1 group and high calorie diet 2 group, and no significant difference in basic diet, high protein and high fat group with equal energy is observed. The expression of UCP2 mRNA between brown adipose tissue and muscle is simliar in all groups. Conclusion Energy can induce UCP2 mRNA expression in rat white adipose tissue, and expression level is related to store energy in body, but the expression of UCP2 mRNA in brown adipose tissue and muscle is unrelated to diet composition.