共轭亚油酸诱导人乳腺癌细胞(MCF-7)凋亡的作用

Effect of apoptosis in human mammary cancer (MCF-7) cells induced by cis9,t11-conjugated linoleic acid

目的 为了研究共轭亚油酸单体 (c9,t11 CLA)对人乳腺癌细胞 (MCF 7)凋亡的影响。方法 采用细胞生长曲线 ,凋亡细胞的荧光显微镜和电镜观察、流式细胞光度术的检测以及p5 3蛋白表达的方法 ,观察了用不同浓度c9,t11 CLA(2 5、5 0、10 0和 2 0 0 μmol L)诱导MCF 7细胞凋亡作用。结果 c9,t11 CLA可明显抑制MCF 7细胞生长 ,8天的抑制率分别为 -6 0 %、4 5 2 %、99 0 %和 99 4 %。通过荧光显微镜和电镜技术观察到了发生凋亡的MCF 7细胞 ;用流式细胞光度术也检测到c9,t11 CLA可诱导MCF 7细胞产生凋亡 ,并随着c9,t11 CLA浓度的增加 ,细胞凋亡率逐渐增加 ;而p5 3蛋白表达则随着c9,t11 CLA浓度的增加呈现逐渐降低的趋势。结论 c9,t11 CLA可诱导MCF 7细胞产生凋亡 ,这可能是c9,t11 CLA抑制肿瘤细胞生长的机制之一。

Objective To determine the effect of cell apoptosis induced by c9,t11-conjugated linoleic acid was investigated in human mammary cancer cells (MCF-7).which were treated with various c9, t11- CLA concentrations (25,50,100 and 200μmol/L), with a negative control. Methods In this study, the methods of the curve of cell growth, cell apoptosis detecting in fluorescent and electronic microscope and flow cytometry, and expression of p53 protein were employed. Results The cell growth of MCF-7 cells was inhibited by c9,t11-CLA. Eight day after treatment with various concentrations of c9,t11-CLA, as mentioned above, the inhibition rates were -6.0%, 45.2%, 99.0% and 99.4%, respectively. Apoptotic cells of morphology from MCF-7 cells treated by different concentrations of c9,t11-CLA were observed by fluorescent and electronic microscope and the frequency of apoptosis in MCF-7 cells by flow cytometry showed increasingly with concentrations of c9,t11-CLA increased. While the expression of p53 protein in MCF-7 cells was decreased with the doses of c9,t11-CLA increased. Conclusion c9,t11-CLA can induce the effect of apoptosis in MCF-7 cells and its may be one of mechanisms for the inhibitory effect of c9,t11-CLA in human cancer cells.