锌、硒对糖尿病大鼠糖、脂代谢紊乱的调节及其分子机制的研究

Effects of zinc and selenium on the disorders of blood glucose and lipid metabolism and its molecular mechanism in diabetic rats

目的 为研究锌、硒对糖尿病大鼠糖、脂代谢的影响及其分子机制。方法 于补充锌、硒前后检测各组大鼠空腹血糖、血清胰岛素及血脂 ;运用mRNA差异显示技术观察各组大鼠之间肝脏中基因表达的变化。结果 糖尿病补锌组 (DM +Zn)、糖尿病补硒组 (DM +Se)大鼠血糖水平较实验前明显降低 ;血清总胆固醇、甘油三酯、低密度脂蛋白胆固醇、动脉硬化指数明显低于糖尿病对照组 (DM) ,高密度脂蛋白胆固醇高于糖尿病对照组 ;血清胰岛素水平明显改善。分离到糖尿病补锌组、补硒组与糖尿病对照组之间出现明显差异的 4 0 0bp以上的cDNA片段 31条 ,其中在补锌组、补硒组中高表达的cDNA片段 19条 ;在糖尿病对照组中高表达的cDNA片段 12条。结论 锌、硒对糖尿病大鼠糖、脂代谢紊乱具有一定的改善作用 ;对其某些基因的表达产生了诱导或抑制作用。锌、硒对糖尿病大鼠代谢紊乱的调节作用可能与这些基因表达水平的变化有关。

Objective In order to study the effects of zinc and selenium on glucose and lipid metabolism and its molecular mechanism in diabetic rats.Methods The Blood glucose, lipid and serum insulin were measured before and after the treatment, and the changes in gene expression among four groups were studied by mRNA differential display technique.Results The blood glucose in DM+Zn group, DM+Se group decreased significantly than that before experiment; the levels of serum TG, TC, LDL-c and AI in DM+Zn group and DM+Se group were lower than those of DM group, while the serum HDL-c levels were higher. The serum insulin was also improved obviously. 31 cDNA fragments larger than 400bp expressed differences in liver between DM+Zn group, DM+Se group and DM group were isolated, 19 of them expressed higher in DM+Zn group and DM+Se group, while the rest expressed higher in DM group. Conclusion It is suggested that zinc and selenium supplementation could partially improve the disorders of glucose and lipid metabolism, and have an impact on some gene expression in diabetic rats, which may contribute to the regulating effects on its disorders of metabolism.