家蝇幼虫中与抗菌功能相关的蛋白和多肽的分析

Analysis of antibacterial-relative proteins and peptides in housefly larvae

目的 研究家蝇幼虫体内与抗菌功能相关的蛋白和多肽。方法 对家蝇 3龄幼虫进行针刺、带菌针刺、热激、超声 4种处理 ,于处理后 6、16、2 4、4 8、6 5h分别收集提取耐热水溶蛋白 ,比浊法测定抗菌活性 ,经逐步回归分析确定抗菌相关蛋白和多肽。结果 4种处理均能诱导家蝇幼虫产生抗菌物质 ,其中表观分子量为 2 0kDa、2 2kDa的蛋白对金黄色葡萄球菌和枯草杆菌均有抗菌作用 ,13kDa、16kDa、2 6kDa蛋白只对上述两种菌中的一种有作用 ,37kDa、4 4kDa的蛋白对抗菌作用具有显著负贡献。结论 家蝇幼虫经过诱导后 ,会有多种与抗菌活性相关的蛋白和多肽开始表达或加强表达 ,它们彼此合作 ,共同建立一个完整的防御体系。

Objective To study the antibacterial-relative proteins and peptides in housefly larvae. Methods Third-instar larvae of housefly were treated with different methods including pricking, pricking with bacteria, heating and ultrasonication and were extracted respectively after 6h,16h,24h,48h,65h. After liquid phase assay, the antibacterial-relative proteins and peptides were confirmed with stepwise regression analysis. Results The four treatments could all induce antibacterial substances. Among these induced proteins, X 17(20kDa), X 16(22kDa) had activity against Staphylococcus aureus and Bacillus subtilis, but X 22(13kDa), X 20(16kDa), X 12(26kDa) had activity only against either Staphylococcus aureus or Bacillus subtilis. X 7(37kDa),X 5(44kDa) had negative contribution to antibacterial activity. Conclusion Many antibacterial-relative proteins and peptides which cooperate and establish a whole resisting system will begin to be translated or be translated more.