甘草化学成分的高效液相色谱-串联质谱分析

Rapid Identification of Compounds in Glycyrrhiza Uralensis by Liquid Chromatography/Tandem Mass Spectrometry

采用高效液相色谱 质谱联用方法分析了甘草 (Glycyrrhizauralensis)中的化学成分。通过高效液相色谱可将三萜、黄酮及香豆素等 5 0余种化学成分较好的分离。根据紫外光谱可大致判断其化合物类型 ,由电喷雾质谱得到各成分的分子量 ,再由串联质谱获得进一步的结构信息 ,进而推测出其中 2 2个主要成分的可能结构。它们分别是 :葡糖基甘草甙、异光果甘草甙、夏拂托甙、甘草素 4′ 芹糖甙 ,甘草甙 ,6′ 乙酰甘草甙 ,异佛来心甙 ,异甘草素葡萄糖芹菜甙 ,甘草糖甙A ,甘草糖甙B ,甘草素 ,3 O [β D 葡萄糖醛酸甲酯 (1→ 2 ) β D 葡萄糖醛酸 ] 2 4 羟基 甘草内酯 ,甘草皂甙E2 ,异甘草素 ,甘草醇 ,甘草酸 ,甘草香豆素 ,甘草双氢异黄酮 ,甘草异黄酮甲 ,乌拉尔甘草皂甙乙 ,新甘草酚和甘草皂甙B2 。

The chemical constituents of Glycyrrhiza uralensis were first analyzed by high performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS n). Above fifty major components, including triterpenoid saponins, flavonoids and coumarins, were isolated. Among them, twenty-two were identified as glucoliquiritin asioside, iosliquiritoside, schaftoside, liquiritigenin-4′-apiosyl(1→2)-glucoside, liquiritin, 6′-acetyliquiritin, isoviolanthin, licuraside, licorice-glycoside A, licorice-glycoside B, liquiritigenin, 3-O-[β-D-(6-methyl)glucuronopyranosyl(1→2)-β-D-glucuronopyranosyl]-24- hydroxy-glabrolide, licorice-saponin E 2, isoliquiritigenin, glycyrol, glycyrrhizic acid, glycycoumarin, licoisoflavanone, licoisoflavone A, uralsaponin B, neoglycyrol and licorice saponin B 2 by the ESI-MS, ESI-MS n and ultraviolet (UV) spectra.