新型超支化聚合物涂层毛细管电泳柱的制备及评价

Preparation and Evaluation of Hyperbranched Polymer Coated Column for Capillary Electrophoresis

以 1,1,1 三羟甲基丙烷、丙烯酸甲酯和二乙醇胺为原料合成出超支化聚 (胺 酯 ) ,通过物理吸附方法将其涂布于熔融毛细管柱内表面 ;该方法制备的涂层柱能有效地抑制电渗流和蛋白质在毛细管壁的吸附作用 ;在pH 5 .0的缓冲液中 ,碱性蛋白质的平均柱效为 5 .1× 10 5理论塔板数 /m 。

The hyperbranched poly(amine-ester) based on 1,1,1-trimethylolpropane, methyl acrylate and diethanolamine was synthesized and coated on the inner surface of the fused capillaries by physical adsorption. The coated column prepared by this method was good enough to repress the electroosmotic flow and the adsorption of the proteins on the capillary wall. The average separation efficiencies were 5.1×10 5 plates/m and the coating had a good stability for basic proteins using the buffer of pH 5.0.