一种毛细管电泳-化学发光联用系统的改进及应用

Improvement and Application of A Capillary Electrophoresis-Chemiluminescence System

在毛细管电泳 化学发光联用研究工作的基础上 ,提出了一种简单可靠的联用检测接口 ,并对两端电极池作了改进。高电位池的改进 ,解决了支持缓冲液中鲁米诺电解造成的发光信号漂移问题 ;低电位池的改进 ,使发光反应中排出的废液不混入电极缓冲溶液。从而保持化学发光信号的基线稳定 ,避免发光试剂的损耗并延长缓冲溶液的使用时间。整个体系简单易行 ,采用此联用系统和鲁米诺 铁氰化钾间接化学发光法 ,分离了绿原酸和芸香苷。本法的检出限达到 1.4× 10 -5mol/L ,比毛细管电泳 紫外吸收法降低约

A simple and reliable capillary electrophoresis-chemiluminoscnece (CE-CL) system with improved buffer reservoirs was developed. The amelioration of the high potential buffer reservoir can overcome the CL signal excursion, which resulted from the electrolysis of luminol in running buffer. The improvement of the low potential buffer reservoir can prevent the contamination of electrode buffer from the CL reaction waste. As a result, the CE-CL system can retain the stability of CL baseline, avoid the excessive consumption of the CL reagent and prolong the working time of the running and electrode buffer solution. The proposed CE-CL system was adopted for the separation and determination of chlorogenic acid and rutin with the indirect chemiluminescence of luminol-potassium ferricyanide. The detection limit of CE-CL is 1.4×10 -5 mol/L, which is about 2 order of magnitudes better than that of CE-ultraviolet detection.