絮凝酵母SPSC01连续培养最适生长条件的研究

Optimization of Growth Parameters During the Continuous Culture of Flocculating Yeast Strain SPSC01

采用均匀设计实验法对气升式内环流生物反应器中絮凝酵母SPSC0 1的高密度培养条件进行了研究 ,确定了各因素与目标函数之间的关系 ,并通过综合调优 ,获得该菌株的最佳培养条件 :温度 30℃ ;通气量 0 71 4vvm ;培养基组成为 :双酶法制备的玉米糖化液 ,糖浓度为 2 2 0g L ,添加 4mL L玉米浆和 3g L(NH4 ) 2 HPO4 ;稀释速率控制为0 0 2h- 1 。在上述条件下进行絮凝酵母的连续培养 ,培养液中的酵母细胞密度达到了 2 0g(d .w)

Using uniform design principles, the continuous culture of flocculating yeast strain SPSC01, a fusant from Schizosaccharomyces pombe and Saccharomyces cerevisiae was investigated in an air lift suspended bed bioreactor with a working volume of 1400mL. The optimum culture conditions for this flocculating yeast strain were obtained as follows: temperature, 30℃; aeration rate, 0 714 vvm; medium (based on corn powder two step enzymatic hydrolysis) contained sugar of 220 g/L, corn steep liquor of 4 mL/L and (NH 4) 2HPO 4 of 3 g/L. As high as 20 g(d.w)/L yeast cells was achieved in the broth for the continuous culture system when the dilution rate was controlled at 0 02 h -1 .