血清淀粉样蛋白A在百草枯中毒致小鼠急性肺损伤中的表达及意义

Significance of serum amyloid A expression in a murine model with paraquat-induced acute lung injury

目的:观察血清淀粉样蛋白A1(SAA1)在百草枯中毒小鼠急性肺损伤的表达及意义。方法:SPF级健康雄性昆明小鼠随机分为对照组和中毒组。中毒组予一次性腹腔注射百草枯(20 mg/kg)复制染毒小鼠模型,对照组予注射等剂量生理盐水,24 h后处死小鼠并取材。检测小鼠肺湿/干重比,苏木精-伊红染色观察肺组织病理变化,原位末端缺刻标记法观察细胞凋亡,酶联免疫吸附法检测小鼠血清SAA的表达情况,免疫荧光定量PCR法检测小鼠肺组织TGF-β1 mRNA表达的情况。结果:与对照组相比,中毒组小鼠肺组织湿/干重比、肺组织炎症及细胞凋亡显著增加,血清SAA含量明显增多(P<0.05);肺组织TGF-β1 mRNA的表达均增高。结论:SAA在百草枯中毒小鼠肺损伤中显著增高,参与其炎症过程,并在其病程进展中发挥重要作用。

Objective:To investigating the expression and significance of serum amyloid A(SAA)in paraquat-induced acute lung injury(ALI)in a murine models.Method:SPF healthy Male Kunming mice were randomly divided into the control group and the PQ poisoned group.The PQ poisoning group was given a one-time intraperitoneal injection of paraquat(20 mg/kg)to replicate the mouse model,and the control group was adopted the normal saline solution with the same volume.After 24 hours,the mice were sacrificed and samples were collected.The edema of lung tissue were measured by lung wet/dry weight ratio(W/D),the histological changes and apoptosis were observed by HE staining and TUNEL staining,serum level of SAA1 was assayed by ELISA,the mRNA expression of TGF-β1 mRNA in the lung tissue were detected by real time-PCR assay.Result:The lung inflammation,apoptosis,W/D ratio and the level of SAA1 in the serum of PQ-induced mice were significantly increased compared to the control group.The lung parenchyma TGF-βmRNA were significantly expressed in the PQ poisoning group.Conclusion:SAA1 was strongly linked to the development of PQ-induced ALI and played a important role in the disease progression of ALI induced by paraquat.