LncRNA KCNQ1OT1在DLBCL患者中的表达及其临床意义

Expression of LncRNA KCNQ1OT1 in DLBCL patients and its clinical significance

目的 :探讨长链非编码RNA(long non-coding RNA,LncRNA )KCNQ1重叠转录物1(KCNQ1 overlapping transcript 1,KCNQ1OT1)在弥漫大B细胞淋巴瘤(di use large B cell lymphoma,DLBCL)患者外周血中的表达及其临床意义。方法 :采用实时荧光定量PCR法检测120例DLBCL患者和55例非肿瘤患者(作为对照组)血清中LncRNA KCNQ1OT1的表达,分析其与DLBCL患者临床病理特征及预后的关系。结果 :DLBCL患者血清中LncRNA KCNQ1OT1的表达水平显著高于对照组(P <0.001)。LncRNA KCNQ1OT1的表达与肿瘤大小、Ann Arbor分期、B症状、化疗敏感性及淋巴瘤国际预后指数(international prognostic index,IPI)均有关(P值均<0.001)。LncRNA KCNQ1OT1高表达的DLBCL患者无进展生存及总生存时间较低表达者均明显缩短(P值均<0.001)。Ann Arbor分期、LncRNA KCNQ1OT1表达、淋巴瘤IPI及化疗敏感性是DLBCL患者预后的独立影响因素(P值均<0.01)。结论:LncRNA KCNQ1OT1在DLBCL患者外周血中高表达,并且是DLBCL患者预后的独立影响因素。

Objective:To investigate the expression of long non-coding RNA(LncRNA)KCNQ1 overlapping transcript 1(KCNQ1OT1)in the peripheral blood of patients with diffuse large B-cell lymphoma(DLBCL),and to analyze its clinical significance.Methods:The expression of LncRNA KCNQ1OT1 in the serum samples of 120 DLBCL patients and 55 non-tumor patients(as the control group)was detected by real-time fluorescent quantitative PCR.The correlations of LncRNA KCNQ1OT1 expression with the clinicopathological features and prognosis of DLBCL patients were analyzed.Results:The expression level of LncRNA KCNQ1OT1 in the serum of DLBCL patients was higher than that in the control group(P<0.001).The expression of LncRNA KCNQ1OT1 was correlated with the tumor size,Ann Arbor stage,B symptoms,chemosensitivity and the international prognostic index(IPI)(all P<0.001).The progression-free survival(PFS)time and the overall survival(OS)time of DLBCL patients with LncRNA KCNQ1OT1 high expression were significantly shorter than those with LncRNA KCNQ1OT1 low expression(both P<0.001).Ann Arbor stage,LncRNA KCNQ1OT1 expression,IPI and chemosensitivity were the independent prognosis factors for DLBCL patients(all P<0.01).Conclusion:LncRNA KCNQ1OT1 is highly expressed in the serum of patients with DLBCL,and is one of independent prognosis factors for DLBCL patients.