防虫磷对杂拟谷盗成虫体内乙酰胆碱酯酶抑制作用的研究

Research in the Inhibiting of Fang chong Lin on AchE in Adult Worms of Tribolium confusum Jacquclin du Val

本文作者用酸碱滴定法、比色法研究了防虫磷(纯度97%)对杂拟谷盗(Tri-bolium confusum Jacquclin du Val)成虫体内AchE的抑制效应.结果表明:抑制剂防虫磷对虫体内AchE产生的抑制反应,依赖于足够的抑制剂剂量.当剂量按梯度的增加,杂拟谷盗的击倒率及其体内AchE的抑制率亦随着增加,而AchE的剩余活性相应地减弱.防虫磷对虫体内AchE的抑制作用,符合一级反应动力学特征.从中获得的Ki值及PI_(50)能灵敏地反映抑制剂的作用程度.同时能准确地衡量AchE对抑制剂敏感性的强弱变化,为此,应列入储粮害虫抗性鉴测及抗性品系确证的重要指标.

This paper reviews the way how Fang chong Lin has an effect on Ac-h E in bodies of adult worms of Tribolium coniusum Jacquclin du Val. Using a neutralizung method and colorimetric method, the result shows that the inhibitor Fang chong Lin can produce an inhibiting effect on the worms only under the condition in which enough dosage of the inhibitor is used. Both the rates of knock-down of the worms and inhibition of Ac hE in the bodies will increase with the gradients of the dosage, but the remaining activity of AchE decreases instead. The reaction of the inhibitor of Fang chong Lin on adult worms is in conformity with the characteristic of the reaction of dynamics Grade I. The data of Ki and PI50 derived from the above can sensitively show the extort of reaction by the inhibitor and exactly judge the sensitive changes in the effectiveness of the inhibitor on AchE. I suggest the derived Ki and PI50 shoud be listed as an important indicalor to monitor and confirm the resistance of insects