小麦赤霉病菌FgVPS26与FgRab7的相互作用及对DON毒素合成的影响

Interaction between FgVPS26 and FgRab7 and Their Effect on the Synthesis of DON Toxin in Fusarium Graminearum

小麦赤霉病菌合成DON毒素是多基因控制的次级代谢过程,阐明各种基因的调控作用有利于进行科学防控。以赤霉病菌为研究对象,通过酵母双杂交、实时定量PCR及ELISA等技术研究FgVPS26与FgRab7的相互作用及对DON毒素生物合成的影响。结果表明:FgVPS26和激活态FgRab7可以相互作用,但与原始态FgRab7不能相互合作。FgVPS26和FgRab7基因可相互影响其表达,与野生型菌株PH-1相比,当FgVPS26和FgRab7其中一个基因缺失时另一个基因的表达量将显著下降。另外,FgVPS26或FgRab7基因缺失也显著降低与DON毒素合成相关的Tri基因表达,从而导致同一生长时期突变菌株的DON毒素合成量比野生型菌株PH-1明显减少。因此,小麦赤霉病菌中FgVPS26和FgRab7可以相互作用并调控DON毒素的生物合成。

The biosynthesis of deoxynivalenol(DON)toxin is a secondary metabolic process regulated by multiple genes in Fusarium graminearum,and elucidating the regulatory roles of the related genes is conducive to the development of scientific prevention and control measures.In this study,yeast two-hybrid,real-time quantitative PCR and ELISA were employed to investigate the interaction between Fg VPS26 and Fg Rab7 and their effect on the biosynthesis of DON toxin.The results showed that Fg VPS26 could interact with activated Fg Rab7,but could not interact with the original state of Fg Rab7.The expression of Fg VPS26 and Fg Rab7 genes could influence each other,and when one of the genes(Fg VPS26 and Fg Rab7)was knocked out,the expression of the other gene decreased significantly compared with the level in wild-type strain PH-1.Additionally,deletion of Fg VPS26 or Fg Rab7 gene also significantly affected the expression of Tri gene involved in DON toxin synthesis,which resulted in the decrease of DON toxin synthesis of mutant strains compared with that of wild type strain at the same growth stage.Our results suggested that Fg VPS26 and Fg Rab7 could interact with each other and regulate the biosynthesis of DON toxin in Fusarium graminearum.