人胆结石的中枢神经药理作用的实验研究

A Study of Pharmacodynamic Effects of Calculus Felleus Humaneus

本实验探索了人胆结石对中枢神经的药理作用。结果:400mg/kg 人胆结石可减少小鼠自主活动;延长戊巴比妥钠的催眠时间;能对抗戊四氮致阵孪性惊厥;降低酒石酸锑钾致小鼠扭体反应数。600mg/kg 人胆结石连续口服5天能对抗电惊厥作用。100mg/kg 人胆结石能使三联菌苗致发热家兔体温下降。初步表明人胆结石具有一定的安定、镇惊、清热、镇痛等中枢神经药理作用。和天然牛黄、人工牛黄类似,具有一定的药用价值。

Based on Li Shizhen's discription of the bile as a drug in his"Compendium of Materia Medics",we conducted experiments to study the pharmacodynamic effects of human gallstone(Calculus Felleus Humaneus,CFH)obtained in the operation room,Calculus Bovis from Australia and factitious Calculus Bovis on the nerve centres of mice,including:(1)effects on the active movements of mice;(2)hypnogenic synergism with pentobarbital;(3)anticonvulsive effect against pentylenetetrazol;(4)anticonvulsive effect against electricity;(5)effect on vaccinum typho-paratyphosum fever;(6)analgesic effect(by van der Wende's experiment and Woolfe-Macdonald's experiment). As a result of the experiments it was found that with intraperitoneal administration of CFH(400mg/kg) the spontaneous activity of mice could be obviously restrained(P<0.01),the period of hypnotism caused by pentobarbital(20mg/kg)could be prolonged(P<0.05),the spasmodic convulsion caused by pentylenetetrazol (50mg/kg)could be counteracted(P<0.01)and twist reflex caused by potassium antimony tartrate(0.02%, 0.2ml/10g)could also be abolished(P<0.01),but the time of Woolfe-Macdonald's reaction could not be lengthened.Oral administration of CFH(60mg/kg)for five days can effectively prevent electric convulsion. With a dose of 100mg/kg,it can lower the high body temperature of rabbits induced by vaccinum typho- paratyphosum(0.8ml/kg).These results showed that CFH had sedative,anticonvulsive,antifebrile and analgesic effects on the animal nerve centres.It had some pharmacodynamic effects similar to those of Calculus Boris and factitious Calculus Bovis.It may have some practical value in therapeutics in the future.