老年高血压病患者中医证型与IL-6基因rs1800796位点多态性的相关性研究

Correlation Study between Chinese Medicine Syndrome and Interleukin- 6 rs1800796 Gene Polymorphisms in Elderly Hypertensive Patients

目的调查上海市老年高血压病患者证型分布规律并探讨中医证型与IL-6基因rs1800796位点多态性之间的相关性。方法 2013年3—12月收集上海市4个社区老年高血压病患者651例,并进行辨证分型,收集患者一般资料。采集患者血样,分析IL-6基因rs1800796位点基因多态性与中医证型相关性。结果 651例老年高血压患者证型分布依次为阴虚阳亢证(211/651,32.41%)、痰瘀互结证(156/651,23.97%)、肝火亢盛证(154/651,23.66%)、肾气亏虚证(130/651,19.97%)。不同证型间男女比例不同(P<0.01),男性患者肝火亢盛证(87/285,30.53%)为主要证型,女性患者阴虚阳亢证(135/366,36.89%)为主要证型。老年高血压病患者IL-6基因rs1800796位点基因型分布依次为CC(329/651,50.54%), GC(280/651,43.01%),GG(42/651,6.45%)。不同证型之间IL-6基因rs1800796位点基因型分布差异有统计学意义(χ~2=18.457,P=0.005),痰瘀互结证GC型为主要基因型,其余证型CC型为主要基因型。结论老年高血压病IL-6基因rs1800796位点基因型与中医证型存在一定的关联性,IL-6基因rs1800796位点GC型有可能是老年高血压病痰瘀互结型的易感基因。

Objective To investigate the distribution of Chinese Medicine(CM)syndrome in Shanghai elderly hypertensive patients and to explore the correlation between CM syndrome and interleukin-6(IL-6)rs1800796 gene polymorphism.Methods From March 2013 to December 2013,totally 651 elderly hypertensive patients were recruited from four communities in Shanghai.The patients were classified according to syndrome differentiation,their general information and blood samples were collected.The correlation between IL-6 rs1800796 gene polymorphism and CM syndromes were analyzed.Result The syndrome type distribution in 651 elderly hypertensive patients were sequenced as yin deficiency and yang excess syndrome(211/651,32.41%),phlegm-stasis syndrome(156/651,23.97%),Gan-fire excess syndrome(154/651,23.66%),Shen qi deficiency syndrome(130/651,19.97%).The proportion of male and female patients with different syndromes was different(P<0.01).In male patients,the main syndrome was Gan-fire excess(87/285,30.53%).In female patients,the main syndrome was yin deficiency and yang excess(135/366,36.89%).In elderly hypertensive patients,the genotype of IL-6-rs1800796 was sequenced as CC(329/651,50.54%),GC(280/651,43.01%),GG(42/651,6.45%).The genotype distribution of IL-6 rs1800796 between different syndromes were different(χ~2=18.457,P=0.005).The main IL-6-rs1800796 genotype of phlegm-stasis syndrome was GC,and the main IL-6-rs1800796 genotype of other three syndromes was CC.Conclusions There is a certain correlation between elderly hypertension and IL-6-rs1800796 genotype.IL-6-rs1800796 GC type may be a susceptibility gene for phlegm-stasis syndrome in elderly hypertension.