七彩红竹MYB转录因子基因的克隆与分析

Cloning and Sequence Analysis of MYB Transcription Factor Gene from Indosasa hispida cv. ‘Rainbow'

MYB基因是最大的植物转录因子家族成员之一,在植物次生代谢、生长及发育过程中发挥着重要作用。利用RACE方法,从七彩红竹中克隆得到1个与MYB同源的基因,命名为Ih MYB4,并利用RTPCR检测Ih MYB4在不同组织部分表达情况。序列分析表明:Ih MYB4序列全长1044 bp,编码347个氨基酸,该蛋白含有2个MYB功能结构域,属于R2R3-MYB家族。系统进化分析表明:Ih MYB4蛋白可能是一类参与花青素生物合成调控相关的蛋白。RT-PCR检测表明Ih MYB4只有在微红的幼嫩竹秆中才表达,说明Ih MYB4参与七彩红竹中花青素生物合成的调控。

As one member of the largest transcription factors family,MYB gene plays an important role in the processes of second metabolisms and growth development in plants. A gene which was homologous with MYB was cloned from Indosasa hispida cv. ‘Rainbow'by the rapid amplification of c DNA ends( RACE) method,and named as Ih MYB4. The Ih MYB4 expression in different organs of I. hispida cv. ‘Rainbow ' was tested by RT-PCR. The sequence analysis showed that the c DNA sequence of Ih MYB4 consisted of 1044 bp encoding347 amino acid,and contained two Myb-DNA-binding domains,indicating that it was belonged to the R2R3-MYB family. Phylogenetic analysis,based on protein sequence,showed that the Ih MYB4 protein was likely to be involved in the regulation of anthocyanins biosynthesis. The Ih MYB4 was expressed only in young red culm by RT-PCR,suggesting that the Ih MYB4 participated in the regulation of anthocyanins biosynthesis of I. hispida cv. ‘Rainbow'.