运动训练后大鼠脑梗死灶周围HMGCoAr水平的变化

The changes of HMGCoAr level in region around infarction area in rats with acute cerebral infarction after mobility training

目的探讨运动训练后大鼠脑梗死灶周围3-羟-3-甲戊二酰辅酶A还原酶(HMGCoAr)的表达水平变化和大鼠运动功能恢复的关系。方法将大脑中动脉闭塞致脑梗死的90只大鼠模型用随机数字表法分为训练组、他汀组和对照组,每组各30只,对训练组和他汀组进行运动训练;在制模后的3、7、14 d用横木行走试验评定大鼠运动功能;分别用半定量逆转录-多聚酶链反应和免疫组织化学法测定每组大鼠脑梗死灶周围组织的HMGCoAr-mRNA及其蛋白表达水平。结果运动训练后训练组和对照组大鼠运动功能评分均增高,3、7和14 d 3次评分均有明显差异(P<0.05);在制模后7、14 d,训练组的运动功能评分值分别为(5.7±0.2)和(6.8±0.4)分,均高于对照组的(3.6±0.2)和(5.1±0.3)分(P<0.05);他汀组运动功能评分在以上3个时间点间比较均无明显差异(P>0.05),并在7、14 d均低于训练组和对照组的评分(P<0.05);3组脑梗死大鼠病灶周围HMGCoAr-mRNA(吸光度)和蛋白[阳性信号密度值(%)]水平均为14 d>7 d,7 d>3 d(P<0.05);其中他汀组指标(HMGCoAr-mRNA:0.591±0.024,0.764±0.017,0.894±0.012;蛋白:0.423±0.016,0.519±0.008,0.687±0.001)均>训练组(HMGCoAr-mRNA:0.416±0.043,0.654±0.042,0.765±0.036,蛋白:0.301±0.022、0.425±0.014、0.592±0.003),而后者的相关指标水平也均>对照组(P<0.05)。结论运动训练可使大鼠脑梗死灶周围HMGCoAr-mRNA及其蛋白表达水平增高,并有利于恢复运动功能。

Objective To investigate the effects of mobility training on the mobility skill and the level of 3-hydroxy-3-methylglutaryl coenzyme A reductase(HMGCoAr)in the region around the infarction area in rats with acute cerebral infarction.Methods Ninety models of rats with cerebral infarction of middle cerebral artery occlusion were randomly divided into 3 groups:the train group,the mevastatin group,and the control group.The rats in the train group and the mevastatin group received motor skill training.The motor skills were examined in the 90 rats via beam walking test at the 3rd,the 7th and the 14th days after the model was made.The levels of both mRNA and protein of HMGCoAr in the region around the infarction were observed through RTPCR and Western blot,respectively.Results After mobility training,the mobility skill of both the train group and the control group were significantly higher at 14th day than that at 7th day,and higher at 7th day than that at the 3th day(P<0.05);The scopes were higher in the train group(5.7±0.2,6.8±0.4)than those in the control group(3.6±0.2,5.1±0.3)at the 7th day and 14th day,respectively.The scopes of both groups were significantly higher than those of the mevastatin group at the both time points.However,there was no significant difference among the scopes of mobility skill of the mevastatin group at the three times.Meanwhile,the levels of both mRNA(absorbance)and protein[density values of Positive signal(%)]of HMGCoAr in the region around the infarction area of the three groups were significantly higher at S U 14th day than those at the 7th day,and higher at the 7th day than those at the 3th day(P<0.05).The scopes were statistically higher in the mevastatin group(HMGCoAr-mRNA:0.591±0.024,0.764±0.017,0.894±0.012;protein:0.423±0.016,0.519±0.008,0.687±0.001)than those of the train group(HMGCoAr-mRNA:0.416±0.043,0.654±0.042,0.765±0.036,protein:0.301±0.022,0.425±0.014,0.592±0.003),and the later is higher than those of the control group(P<0.05).Conclusion The motor skill and the levels of mRNA and protein of HMGCoAr in the region around the infarction area could be increased by motor skill training in the experimental rats with acute cerebral infarction.