加味过敏煎对荨麻疹小鼠皮肤肥大细胞脱颗粒的影响

Effects of Modified Guominjian Decoction( 加味过敏煎) on Degranulation of Mast Cells in Larva of Urticaria Model Mice

目的探讨加味过敏煎治疗荨麻疹的可能作用机制。方法 60只BALB/c小鼠随机分为空白组、单纯IgE组、模型组、过敏煎组、加味过敏煎组、氯雷他定组,每组10只。过敏煎组给予过敏煎10. 27 g/(kg·d)灌胃,加味过敏煎组给予加味过敏煎14. 39 g/(kg·d)灌胃,氯雷他定组给予氯雷他定片2. 10 mg/(kg·d)灌胃,模型组、空白组及单纯IgE组给予等体积生理盐水灌胃,每日1次,连续7次。第6日给药后除空白组外其余各组予尾静脉注射抗DNP IgE单克隆抗体0. 5 ml,次日除空白组和单纯IEg组外其余各组小鼠耳部涂抹2,4-二硝基氟苯50μl激发建立荨麻疹动物模型。第8日检测各组小鼠皮肤肥大细胞脱颗粒情况及蛋白酶激活受体2 (PAR-2) mRNA表达和血清组胺浓度。结果显微镜下观察,空白组与单纯Ig E组未见明显肥大细胞脱颗粒,模型组肥大细胞脱颗粒明显,各给药组肥大细胞脱颗粒数均较模型组低,加味过敏煎组脱颗粒数相对较少。与空白组、单纯IgE组比较,模型组肥大细胞脱颗粒数、PAR-2 mRNA及组胺表达明显增加(P <0. 05或P <0. 01);与模型组比较,加味过敏煎组肥大细胞脱颗粒数、PAR-2 mRNA及组胺表达均明显降低(P <0. 01),且均低于过敏煎组与氯雷他定组(P <0. 05或P <0. 01)。结论加味过敏煎可能通过下调皮肤PAR-2 mRNA表达,降低血清组胺释放,进而抑制皮肤肥大细胞脱颗粒,稳定肥大细胞,以达到防治荨麻疹的作用。

Objective To study the possible mechanism of Modified Guominjian Decoction(加味过敏煎)in treating urticaria.Methods A total of 60 BALB/c mice were randomly divided into blank group,simple IgE group,model group,Guominjian Decoction group,Modified Guominjian Decoction group and loratadine group,with 10 rats in each group.The Guominjian Decoction group was given Guominjian Decoction 10.27 g/(kg·d),and the Modified Guominjian Decoction group was given Modified Guominjian Decoction 14.39 g/(kg·d),the loratadine group was given loratadine pills 2.10 mg/(kg·d),and the model group,blank group and simple IgE group were given equal volume normal saline,once a day,for constant 7 days.On the 6 th day,except for the blank group,the other groups were injected with 0.5 ml of anti-DNP IgE monoclonal antibody in the tail vein.The next day,except for the blank group and the simple IgE group,the ears of the other groups were smeared with 24-dinitrofluorobenzene 50μl to activate for the establishment of the animal model of urticaria.On the 8 th day,the degranulation of skin mast cells,the expression of protease activated receptor 2(PAR-2)mRNA and serum histamine concentration were detected in each group.Results With the observation of microscope,there was no obvious degranulation of mast cells in the blank group and the simple IgE group.The degranulation of mast cells in the model group was obvious.The number of degranulation of mast cells in each administration group was lower than that in the model group.The number of degranulation in the Guominjian Decoction group was relatively small.Compared with the blank group and the simple Ig E group,the number of degranulation,PAR-2 mRNA and histamine expression in the model group were significantly increased(P<0.05 or P<0.01).Compared with the model group,the number of degranulation,PAR-2 mRNA and histamine expression in the Modified Guominjian Decoction group were significantly decreased(P<0.01),and were decreased more than those in the Guominjian Decoction group and the loratadine group(P<0.05 or P<0.01).Conclusion The Modified Guominjian Decoction may down-regulate the expression of PAR-2 in the skin,reduce the release of serum histamine,inhibit the degranulation of skin mast cells,stabilize mast cells to achieve the function of treating and preventing urticaria.