通咽喷雾剂对短暂性大脑中动脉栓塞大鼠吞咽功能和肺组织炎症的影响

Effects of Tongyan Spray( 通咽喷雾剂) on Swallowing Function and Pulmonary Inflammation in Rats with Transient Middle Cerebral Artery Occlusion

目的探讨通咽喷雾剂对短暂性大脑中动脉栓塞(t MCAO)模型大鼠吞咽功能和肺组织炎症的影响。方法将70只SD大鼠随机分为空白组10只、模型组30只、通咽喷雾剂组30只。模型组和通咽喷雾剂组采用线栓法阻塞大鼠大脑中动脉,于90 min后再灌,以建立t MCAO大鼠模型。通咽喷雾剂组大鼠在造模后给予通咽喷雾剂口咽部喷雾用药,空白组、模型组代以同等量生理盐水喷雾,每日1次,每次1喷。14天后观察大鼠吞咽潜伏期及吞咽次数,酶联免疫吸附法检测血清、肺组织P物质含量,HE染色观察大鼠肺组织改变,免疫组织化学法观测大鼠支气管黏膜上皮肿瘤坏死因子α(TNF-α)、白细胞介素6 (IL-6)表达。结果与空白组比较,模型组大鼠支气管腔及肺泡充气扩张,肺组织结构紊乱,肺泡间隔变薄;吞咽潜伏期延长、吞咽次数减少,血清、肺组织P物质含量增加,支气管黏膜上皮组织TNF-α、IL-6表达增加(P <0. 05或P <0. 01)。与模型组比较,通咽喷雾剂组大鼠肺组织病变有减轻的趋势,吞咽次数明显增加,血清和肺组织P物质含量增加(P <0. 05或P <0. 01),支气管黏膜上皮TNF-α、IL-6表达无明显改善(P>0. 05)。结论通咽喷雾剂可有效改善t MCAO模型组大鼠的吞咽功能,增加吞咽频率,可能与增加血清、肺组织P物质含量有关,但其对肺组织炎症改变的影响不明显。

Objective To explore the effects of Tongyan Spray(通咽喷雾剂,TY Spray)on swallowing function and pulmonary inflammation in model rats with transient middle cerebral artery occlusion(t MCAO).Methods A total of 70 SD rats were randomly divided into a blank group(n=10),a model group(n=30)and a TY Spray group(n=30).The middle cerebral arteries of rats in the model group and the TY Spray group were blocked by suture method,and reperfusion was given 90 min later to establish the rat model of t MCAO.TY Spray was given to rats in the TY Spray group by oropharyngeal spray after the modeling,while the control group and the model group were given the same amount spray of normal saline,with 1 time per day and 1 spray each time.After 14 days,the latency and number of swallowing were observed.Serum and pulmonary tissue substance P(SP)content was detected by enzyme-linked immunosorbent assay(ELISA),pulmonary tissue changes were observed by HE staining,and tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)expressions in bronchial mucosal epithelium of rats were detected by immunohistochemistry.Results In the model group,the bronchial cavity and alveoli were inflated with gas,the lung tissue was disordered,and the alveolar septa were thinned;the latency of swallowing was prolonged and the number of swallowing was decreased;the SP content in serum and pulmonary tissue was increased;and the expressions of TNF-αand IL-6 in bronchial mucosal epithelial tissue were increased,compared with the blank group(P<0.05 or P<0.01).Compared with the model group,the pulmonary tissue changes of rats in the TY Spray group showed a relieving tendency;the number of swallowing was significantly increased;and the SP content in serum and pulmonary tissue was increased(P<0.05 or P<0.01),without significant improvement in the expressions of TNF-αand IL-6 in bronchial mucosal epithelium(P>0.05).Conclusion Tongyan Spray can effectively improve the swallowing function and increase the swallowing frequency of t MCAO rats,which may be related to the increase of SP content in serum and pulmonary tissue.But it has no obvious effect on the changes of pulmonary inflammation.