喉鳞状细胞癌组织中VEGF和MVD的表达及与颈淋巴结转移的关系

Relationship among expression of the VEGF gene and MVD with cervical lymph nodes metastasis in laryngeal squamous cell carcinoma

目的 :探讨喉鳞状细胞癌 (LSCC)组织中血管内皮生长因子 (VEGF)mRNA及蛋白的表达和微血管密度 (MVD)与颈淋巴结转移的关系。方法 :用半定量逆转录 聚合酶链式反应 (semi RT PCR)技术和免疫组织化学方法检测 6 0例LSCC组织中VEGFmRNA及蛋白的定量表达及MVD。结果 :VEGF的表达无论在基因水平还是蛋白水平伴有颈淋巴结转移组都显著高于不伴有颈淋巴结转移组 (P <0 .0 1)。CD31免疫组织化学染色结果显示伴颈淋巴结转移组的MVD计数显著高于不伴颈淋巴结转移组 (P <0 .0 1)。LSCC组织中MVD计数升高与VEGF高表达呈正相关 (r =0 .94 8 4 ,P <0 .0 5 ) ,且二者与颈淋巴结转移相关。结论 :VEGF表达和瘤内MVD有良好的相关性 ,提示VEGF高表达对LSCC微血管生成起重要作用 ;VEGF高表达和瘤内高MVD与LSCC的颈淋巴结转移密切相关 ,二者有可能成为预测LSCC转移和预后的生物学指标 ;

Objective:To study the relation among the expression of the vascular endothelial growth factor( VEGF) gene and microvessel Density (MVD) with cervical lymph nodes metastasis in laryngeal squamous cell carcinoma(LSCC).Method:Appling semi-RT-PCR technique and immunohistochemistry detected the expression of VEGFmRNA and protein and MVD count in 60 cases with LSCC.Result:The expression of VEGF and MVD was both higher either at molecular level or at the protein level in the group with cervical lymph nodes metastasis than the those with cervical lymph nodes-negtive metastasis. The overexpression of VEGF had a positive correlation with the higher MVD count and were both related to cervical lymph nodes metastasis in the 60 cases with LSCC.Conclusion:There was a significant correlation between the expression of VEGF and MVD, which suggested that the overexpression of VEGF was essential to microangiogensis of LSCC, and the both were related to cervical lymph nodes metastasis and would become a biologic indexes to prognosis of LSCC. Antiangiogenic therapy with specific antibodies to VEGF leads to inhibite angiogenesis and prevent tumor from growth and metastasis, which might have a bright future in clinical application.