中药制剂及外源性细胞因子对化疗损伤小鼠细胞因子及NK细胞活性的影响

Regulatory Mechanism of Traditional Chinese Medicine and Cytokines on the Injury of Chemotherapeutical Mouse

目的研究人参养荣片组方、单味药(人参、当归、黄芪、白术)及单独注射G-CSF对化疗损伤小鼠细胞因子及NK细胞活性的影响。方法酶标双抗体夹心法测定用药后各组小鼠血清中细胞因子(IL-6、IL-12、EPO、G-CSF)含量;小鼠胸腺细胞增殖法(MTT法)测定血清及巨噬细胞培养上清中IL-1含量;NK细胞杀伤靶细胞K562(MTT法)测定NK细胞活性。结果人参可升高血清G-CSF、IL-12,当归可升高巨噬细胞培养上清IL-1及血清IL-12,二者均可增强NK活性;黄芪、白术均可使血清中IL-1、IL-6含量升高。人参养荣片可以将化疗损伤小鼠IL-1、IL-6、IL-12、EPO、G-CSF及NK细胞活性调整至正常水平,但不是单味药作用的累加。注射外源G-CSF后G-CSF、IL-12、NK细胞活性略有升高,IL-1、IL-6、EPO的变化不明显。结论中草药拮抗化疗损伤可能是通过调节多种细胞因子实现的。

Objective To study the cytokines regulatory mechenism of the single (Ginseng, Angelica, Astragalus, Atractyolodes) and complex TCM tablets (RenShenYang-RongPian) and injection G-CSF on the chemotherapeutical mouse. Methods After the different medicine was used to the mouse in different groups. Cytokine's (IL-6, IL-12, EPO, G-CSF) concentration in mouse serum were tested by ELISA, IL-1 content in mouse serum and the cultured supernatants of mononuclear phagocytes were measured by thymocyte proliferation (MTT) method, NK cell activities were determined by NK killed K562 (MTT). Results The G-CSF, IL-12 concentration in mouse serum were increased by Ginseng. The IL-1 in cultured supernatants of mononuclear phagocytes and IL-12 concentration in mouse serum were enhanced by Angelica. Ginseng and Angelica could strengthen the NK cell activities. Astragalus and Atractyolodes could developed the IL-1 and IL-6 levels in mouse serum. The cytokine's (IL-1, IL-6, IL-12, EPO, G-CSF) concentration and NK cell activity of chemotherapeutical mouse would be regulated to the normal level by RenShenYangRongPian, but it wasn't the action together with many of single medicine. After injected the outside G-CSF, the mouse G-CSF, IL-12 concentration and NK activities were increased, but the changes of the mouse IL-1 IL-6 and EPO were not apparent. Conclusion TCM resisted the injury of chemotherapeutical mouse probably by adjusting many kinds of cytokines.