摘要
Objective: To observe human to mouse one way mixed lymphocyte(MLC); And to set up the xeno-grats verse host disease Xeno-graft host disease(XGVHD) model,probing its immunologic mechamism.Methods: Mouse splenic lymphocyte were collected in asepsis and treated by mitomycin as activating cell. Human Peripheral blood lymphocytes (hPBL)were separated and gathered as reacting cell; Mouse splenic lymphocyte and hPBL were mixed to incubate for a week. Destroying recipient (mouse) immune system by total body irradiation (TBI) and intraperitoneal injecting CTX、MTX; Separating and collecting hPBL; Injecting hPBL to mouse by caudal vein. Results; ①HPBL in the experiment groups(mixed mouse lymphocyte) proliferated obviously, the amount of 3H-TdR in corporation increased evidently(P<0.05); The mean percentage of CD 4、CD 8、IgG 、IgM positive cells rose markedly. ②Experiment groups,the hPBL were found in the spleen and kidney tissue, fas protein expressing, we occasionally discovered and apoptosis cells.Conclusion: The human to mouse one-way MLC has obvious lymphocyte proliferation. By these means,we succeed in inducing XGVHD and setting up a XGVHD model.
目的 :①观察人→小鼠单向混合淋巴细胞反应 ;②建立人→小鼠异种移值物抗宿主病 (XGVHD)模型 ,探讨XGVHD的免疫发生机理。方法 :①无菌采集体小鼠脾淋巴细胞 ,经丝裂霉素处理 ,作刺激细胞 ;取健康成人外周血淋巴细胞 (hPBL) ,作反应细胞 ;异种单向混合淋巴细胞培养(one wayXMLC) 1周 ;②摧毁受者 (小鼠 )免疫系统 ;将hPBL经尾静脉注入小鼠体内 ,两周后检测脾、肾组织。结果 :①小鼠脾淋巴细胞明显刺激人外周血淋巴细胞 (hPBL)增殖 ,3 H TdR掺入值 (cpm)显著增高 (P <0 0 5 ) ;CD4 ,CD8,IgG ,IgM细胞含量明显升高 ,有显著差异 (P <0 0 5 )。②实验组 (注射hPBL)动物诱发XGVHD ,脾、肾组织中发现人中CD4、CD8、IgG、IgM细胞 ,有Fas蛋白表达和少量调亡细胞出现。结论 :①人→小鼠单向混合淋巴细胞培养 (one wayXMLC) ,具有明显细胞增殖反应 ;②本实验方法成功诱发XGVHD ,建立人→小鼠异种移殖物抗宿主 (XGVHD)
