摘要
目的 :本文用改进的高效液相色谱 (HPLC)法测定 8位肝癌患者静脉滴注 750mg 5 Fu后 7个时间点的血药浓度。方法 :采用HPLC 紫外检测 (标准曲线法 )。提取 :改用乙腈加异丙醇 ;流动相 :甲醇∶乙腈∶水 (2 5∶5∶70 ) ,内加四丁基溴化铵 ,检测波长 2 65nm。结果 :分离良好 ,完全无血杂质干扰 ;回收率高 (>91 % ) ,日内精密度、日间精密度 <6 % ,监测结果血浓个体差异极大 (2 .5倍以上 )。结论 :本法取血量少 ,提取方法简单 ,离子对试剂效果佳 ,分离良好 ,灵敏度高 ,线性范围宽 (0 .2~2 5 .0mg·L- 1 ) ,适用于基层医院的治疗药物监测 (TDM)。本实验证实 ,5 Fu血浓个体差异较大 ,应该进行TDM ,以实行个体化给药方案 ,使用药更安全、有效、合理和经济。
OBJECTIVE To determine the concentration of 5-Fu in serum at seven time points from 8 Hepatic Cancer patients an by improved method of HPLC. METHODS HPLC-UV Determination was employed.Acetonitrile and iso-propanol were used as the extractive solvent.The mobile phase was methanol-acetonitrile-water(25∶5∶70), tetrabutylamino∶bromide was added.The wavelength for determination was 265 nm.RESULTS Good seperation and high recoveries (>91%) were obtained, without any interference from impurities of serum.Coefficients of variation within a day and between days were less than 6%. The result indicated: individual difference of the drug concentration was significant. CONCLUSIONS Less blood sample has been used by using this method.Extraction method was simple ,ion-pair agent has good spearation.and high sensitivity, good precision and wide liner range ( 0.2 ~ 25.0 mg·L -1 ).It was suitable to determine the concentration of 5-Fu in serum for basic level hospitals. The difference of the concentration of 5-Fu in patients' serum is rather significant.The TDM should be used for the individual administration,which will make the drug administration to be more safe, effective, reasonable and economical.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2004年第2期83-84,共2页
Chinese Journal of Hospital Pharmacy