糖尿病大鼠外周血单个核细胞内与肾皮质中TGF-β_1mRNA水平的相关性研究

TGF-β_1mRNA levels in renal cortex and peripheral blood mononuclear cells of diabetic rats

目的 :观测糖尿病状态及噻唑烷二酮类药物治疗对糖尿病大鼠外周血单个核细胞 (PBMC)内 TGF-β1m RNA水平的影响 ,比较其与肾皮质 TGF-β1基因表达的关系。方法 :5 4只 SD大鼠随机分为正常对照组 (C组 )、糖尿病组 (D组 )及吡格列酮治疗组 (DP组 )。 2、4、8周每组各取 6只 ,以 RT- PCR+狭缝杂交法半定量检测 PBMC内和肾皮质 TGF- β1m RNA水平。结果 :肾皮质 TGF- β1m RNA水平 8周内 D组均高于 C组 (P<0 .0 5 ) ,而 PBMC内TGF- β1m RNA水平 4周时略高于 C组 (1.2 7± 0 .38vs0 .88± 0 .36 ,P>0 .0 5 ) ,8周时差别有统计学意义 (1.12±0 .0 8vs0 .84± 0 .16 ,P<0 .0 5 )。D组和 DP组在 8周时 PBMC内 TGF- β1m RNA水平与肾皮质 TGF- β1m RNA水平呈正相关 (r=0 .83、P=0 .0 2 ,r=0 .82、P=0 .0 3)。结论 :在糖尿病肾病早期 ,PBMC内 TGF- β1m RNA水平增高迟于肾皮质 ;但 PBMC内 TGF- β1m RNA水平可反映肾皮质 TGF- β1m RNA水平的变化。

Objective: To investigate the effect of hyperglycemia and pioglitazone on TGF-β_1 gene expression in peripheral blood mononuclear cells (PBMC) and renal cortex,and the correlation of TGF-β_1mRNA levels between PBMC and renal cortex in STZ induced diabetic rats. Methods: Fifty-four Sprague-Dawley rats were randomly divided into three groups:18 normal control rats (group C),18 diabetic rats (group D) and 18 diabetic rats treated with pioglitazone (20 mg.kg -1 .d -1 , group DP). Six rats from each group were sacrificed at 2,4,8 weeks. TGF-β_1mRNA levels of PBMC and renal cortex were examined by RT-PCR+Slit hybridization analysis. Results: TGF-β_1mRNA level of renal cortex in group D was significantly higher than that in group C at each time point (P<0.05); TGF-β_1mRNA level of PMBC in group D was slightly higher than that of group C at 4 weeks, and significantly higher at 8 weeks (P=0.01). There was positive correlation of TGF-β_1mRNA level between PBMC and renal cortex before (r=0.83,P=0.02) and after pioglitazone treatment at 8 weeks (r=0.82,P=0.03). Conclusion : TGF-β_1mRNA level of PBMC may reflect the change of TGF-β_1 gene expression of renal cortex in diabetic rats.