摘要
本文介绍利用混合纤维素滤膜过滤饮料样品,培养后对其上生长的微菌落进行染色和计数,定量检测饮料中酵母。14份可乐样品在滤膜上的微菌落计数与相应平板培养法计数之间相关关系为(r=0.96,P<0.01),本法迅速,不到12小时报告结果,简便,适于检测污染酵母浓度高于10~3CFU/ml的饮料。
A membrane microcolony count method was developed to measure the amount of yeast in beverages. A specific volume of the beverage sample was filtered by suction through a cellulose mixed membrane filter (pore diameter 5 μm). The filter was placed on the agar media and incubated for a few hours, followed by staining and counting with the light microscope. The amount of yeast in 14 Cola samples were counted by using this method and the plate count method. The results correlated well(γ=0.96, P<0.01). The microcolony count method is rapid, taking less than 12h, simple and suitable for beverages containing above 10~3 CFU yeast per ml.
出处
《现代预防医学》
CAS
1992年第1期8-9,共2页
Modern Preventive Medicine
关键词
微菌落
酵母
饮料
Microcolony Yeast Microscope factor
