缺血缺氧新生鼠海马p-CREB的变化及神经节苷脂对其的影响(英文)

Effects of gangliosides on phosphorylated regulatory element binding protein in CA1 of the hippocampus of neonatal hypoxic ischemic rat

目的:研究缺血缺氧新生大鼠海马结构(主要为CA1)磷酸化c-AMP反应元件结合蛋白质(phosphorylatedcylicAMPresponseelementbindingprotein,p-CREB)变化以及GM1对其的影响。方法:7d龄SD仔鼠共108只,随机分为3组:假手术对照组(36只),缺血缺氧组(HI组,36只)及缺血缺氧+神经节苷脂组(GM1组,36只),于模型建立后1,4,12,24,48和72h处死,免疫组化观察海马CA1区p-CREB的变化。结果:对照组各时间点之间p-CREB的表达差异无显著性意义(F=0.48,P>0.05);HI组、GM1组CA1区p-CREB的表达一过性升高后迅速下降,HI组、GM1组与对照组相比有明显的差异(P<0.05);HI组与GM1相比无明显差异(P>0.05)。结论:缺血缺氧后缺血敏感CA1区p-CREB的表达呈现一过性的升高后迅速下降,GM1不能抑制p-CREB的表达,对神经元的存活没有损害作用,具有较大的临床应用潜能。

AIM:To study the expression of phosphorylated regulatory element binding protein(p CREB) in hippocampus(CA1) of neonatal cerebral hypoxia ischemia(HI,n=36) rat and the effect of gangliosides (GM1). METHODS:108 pieces of SD rats were randomly divided into three groups:control group(n=36),hypoxic ischemia group(HI,n=36),and hypoxic ischemia with GM1 group(GM1,n=36).The level of the p CREB in hippocampus(CA1) were observed respectively at 1,4,12,24,48 and 72 hours after hypoxic ischemia managements. RESULTS:The p CREB levels in CA1 of control group had no difference at different times(P >0.05);the p CREB level in CA1 f HI and GM1 group decreased quickly after a transient increase;there were significant differences between HI group and control group as well as between GM1 group and control group(P< 0.05);there was no significant difference between HI group and GM1 group(P >0.05). CONCLUSION:p CREB level in CA1 of HI group decrease quickly after a transient increase compared with control group after HI management;the expression of p CREB in CA1 is not suppressed by GM1,which is no harm to neurone survival,and therefore with potential clinical applications.