应激对大鼠空间记忆发育的影响(英文)

The effect of stress on spatial memory development in rats

目的:研究应激刺激对发育期大鼠空间记忆发育的影响。方法:采用发育期Wistar大鼠22只,按随机抽签法分为应激1组(30V)、应激2组(45V)、对照组。电刺激大鼠10次/d,连续1周;采用血清放免法检测生长激素含量变化;通过Morris水迷宫检测应激对大鼠空间记忆能力的影响。结果:应激2组水迷宫空间学习记忆逃逸时间较应激1组犤训练第1～5天应激1,2组分别为(23.61±16.06),(14.19±5.01)s犦,较对照组犤训练第1～5天为(26.86±9.89)s犦,差异有显著性意义(t=2.35～3.02,P<0.05);应激2组的生长激素犤(2.24±0.49)μg/L犦含量偏低,应激1组的生长激素含量犤(3.26±0.82)μg/L犦有明显增高趋势(t=2.83,P<0.05)。结论:应激刺激可提高幼年大鼠空间记忆能力的发育;达到45V应激刺激可以影响幼年大鼠的体格发育,而30V应激刺激则不会妨碍甚至可以促进幼年大鼠的体格发育。

AIM: To study the effect of stress on spatial memory development in rats. METHODS: Twenty two Wistar rats at developmental stage were randomly divided into stress group 1,stress group 2 and control group.The rats in stress groups were subjected to electric stress stimulation at the voltage of 30 V and 40 V 10 times daily for a total of 7 days.The changes of growth hormone(GH) contents in blood serum were measured with the radioimmunoassay.The effect of stress on spatial memory was determined through Morris water maze. RESULTS:The escape time of stress group 2 was apparently shorter than that of the stress group 1[(3.61±16.06) and (14.19±5.01) s respectively from the 1 st 5 th day], and was significantly shorter than that of the control group [(6.86±9.89) s](t=2.35-3.02,P< 0.01).The concentration of serum GH decreased below the normal range in stress group 2[(2.24±0.49) μg/L]while it showed a tendency of apparent increase in stress group 1 [(3.26±0.82) μg/L](t=2.83,P< 0.05). CONCLUSION:Stress can promote the development of spatial memory of the developmental rats.When the voltage is higher than 45 V,stress may be harmful to the physical development of the developmental rats; but when the voltage is 30 V. it doesn't inhibit but even benefit the physical development.