甲醛吸入染毒致大鼠多组织器官氧化损伤效应研究

Oxidation damage of formaldehyde inhalation on rat multiple organs

大鼠吸入甲醛 (13 5mg m3 ) ,连续染毒 7d ,每天 4h .染毒结束后 ,测定组织器官 (肺、脑、肝和外周血 )中还原型谷胱甘肽(GSH)含量、谷胱甘肽过氧化物酶 (GSH PX)活力、超氧化物歧化酶 (SOD)活性及脂质过氧化产物丙二醛 (MDA)含量 ,以探讨甲醛对机体的脂质过氧化作用及机体的抗氧化损伤机制 .实验结果表明甲醛吸入组大鼠外周血GSH、GSH PX和MDA水平显著高于对照组 (P <0 0 5 ) ,而甲醛吸入组和对照组相比较 ,大鼠肺、肝、脑组织中的GSH含量、GSH PX活力、SOD活性、MDA含量以及外周血中SOD活性均未见显著性差异 .由此认为 ,外周血抗氧化物GSH、GSH PX活力和脂质过氧化产物MDA水平可望成为甲醛早期暴露的生物效应指标 .

To study the possible mechanisms of oxidative damages in rat multiple organs induced by gaseous formaldehyde, rat was exposed at formaldehyde concentration of 13.5?mg/m^3 for 7 days and 4 hrs per day.Results showed that formaldehyde inhalation caused the significance increase in levels of GSH,GSH-PX and MDA in peripheral of male rat blood.No significant difference could be observed for the concentration of GSH,GSH-PX,MDA and SOD in lung,liver and brain. These results indicate that the level of GSH,GSH-PX and MDA in peripheral blood may be as early exposure biomarkers of formaldehyde in inhalation pathway.