卡那霉素、新霉素对甲基蓝的褪色反应及其分析应用

Fading Reactions of Methyl Blue by Kanamycin, Neomycin and Their Analytical Applications

在pH3～5的条件下,甲基蓝(MB)与硫酸卡那霉素(KANA)、硫酸新霉素(NEO)反应生成离子缔合物,导致MB褪色.最大褪色波长位于608nm(KANA)和606nm(NEO),MBKNNA体系和MBNEO体系的表观摩尔吸光系数(ε)分别为2 44×104L·mol-1·cm-1和2 99×104L·mol-1·cm-1,在一定范围内遵从比耳定律.

In the condition of pH 3.0-5.0, methyl blue (MB) reacts with kanamycin sulfate (KANA) or neomycin sulfate (NEO) to form ion association complexes, which lead to SE fading. The maximum fading wavelengths were located 608 nm(KANA) and 606 nm (NEO); The apparent molar absorptivity (ε) is 2.44×10~4 L·mol^(-1)·cm^(-1) for KANA and 2.99×10~4 L· mol^(-1)·cm^(-1) for NEO; Beer' law is obeyed in definite concentration arange. The method was applied to determine the content of the real sample with satisfactory results.