GFP与微管结合蛋白和肌动蛋白结合区融合蛋白载体构建与表达

Construction of Two Vectors for Expressing the Fusion Genes of GFP with Microtubules Associated Protein 4 and Actin Filaments Talin and Their Expression.

以pCAMBIA1 30 0、pBA0 0 5、pActl D和pMECA等 4个质粒载体为基础 ,根据不同需要 ,选择特定的限制性内切酶作完全或部分酶切 ,通过多步骤的酶切、连接、转化等过程 ,构建完成 2个可通过农杆菌介导转化的植物表达载体。分别命名为 :pActl GFPTALIN和pActl GFP2 MAP4BD。在洋葱表皮细胞中的瞬间表达结果显示 ,两个载体均可正常工作。

Based on plasmid of pCAMBIA1300,pBA005,pAct1 D and pMECA,and digesting the target plasmid DNAs completely or partially with specific single or double restrict enzyme,and from multi cycles of cutting ligation transformation,the construction of pAct1 GFPTALIN and pAct1 GFP2 MAP4BD were finished.they were validated by sequencing and the instantaneous expression of the two fusion genes in surface layer cells of onion with gene gun transformation.;