有效分离1kDa小肽的Tricine-SDS-PAGE方法

An Effective Method of Tricine-SDS-PAGE for Separating the 1kDa Peptide

为了建立能有效分离 1kDa的特小分子肽的Tricine SDS PAGE方法 ,调整分离胶中聚丙烯酰胺凝胶的分子组成 ,使致密胶中丙烯酰胺和双丙烯酰胺的交联度为 5 %,并加入 36 5 %的尿素 ,用于分离小至 1kDa的小分子肽 ,并与常规的SDS PAGE和先前报道的Tricine SDS PAGE相比较。结果改进的致密胶可以有效分离分子量小至 1kDa的小肽 ,明显优于常规的SDS PAGE和现有的Tricine SDS

Current Tricine-SDS-PAGE system is not so effective to resolve the peptide less than 1kDa. Using a different compact gel, which has approximate 5% cross-link of bisacrylamide with acrylamide, to construct modified resolving gel with spacer gel, the peptide with ultra-low molecular mass of 1kDa was resolved effectively.;