鱼腥草新品系离体培养和快速繁殖

Construction of fast propagation system of Houttuynia cordata new line

目的 :通过筛选鱼腥草组织培养最适的植物生长调节剂种类、浓度以及最佳外植体 ,构建起鱼腥草快速繁殖体系。方法 :利用含不同浓度的不同植物生长调节剂种类的MS培养基对鱼腥草新品系不同外植体进行培养。结果 :鱼腥草快速繁殖 ,其外植体以地上茎节和茎尖较适宜。其中 ,MS + 0 .5mg·L- 1KT培养基诱导地上茎节 ,其抽叶数多且植株高。MS + 1.0mg·L- 16 BA培养基则对茎尖愈伤组织诱导作用较佳。MS + 1.0mg·L- 16 BA + 0 .2mg·L- 1NAA和MS + 0 .5mg·L- 16 BA + 0 .2mg·L- 1NAA培养基对地上茎节丛生芽的诱导速度快 ,数目多。生根培养基则以MS + 0 .2mg·L- 1IAA最好。结论 :通过诱导鱼腥草地上茎节丛生芽可大大提高鱼腥草繁殖系数 。

Objective: To construct the fast propagati on system of Houttuynia cordata Thunb. new line by selecting the proper type and c oncentration of plant growth regulartors and proper explants. Method: The different explants of Houttuynia cordata new line were c ultured on MS media with different concentration of different plant grow regul artors. Result: Stem node over-ground and shoot tip were optium explants for fast propagation. The stem which node grew on MS medium with KT at 0.5 mg·L -1 could induce more leaves and grow higher. The shoot ti p cultured on MS medium with 1.0 mg·L -1 6-BA could induce better callus. Adventitious shoots were better achieved from over-ground nodal explants cultu red on MS medium supplemented with 6-BA at 1.0 mg·L -1 and NAA at 0.2 m g·L -1 , or 6-BA at 0.5 mg·L -1 and NAA at 0.2 mg·L -1 . MS medium with IAA at 0.2 mg·L -1 was the best one on inducing roots. Conclusion: The propagat coefficient can be highly improved by in ducing adventitious shoots through stem node over-ground, and thus plentiful s eeds for production can be provided.