高效毛细管电泳法研究口服铝制剂对小鼠血清和脑组织中铝含量的影响

Studies on effects of aluminum compounds on aluminum contents inserum and brain of mice with high performance capillary electrophoresis

目的 :用高效毛细管电泳仪测定小鼠血清和脑组织中铝含量的方法 ,研究口服白矾、氢氧化铝和氯化铝对小鼠血清和脑组织中铝含量的影响。方法 :白矾、氢氧化铝和氯化铝按照铝含量相同的 2个剂量 14 .2 5,57mg·kg- 1·d- 1ig给药 60d后 ,用高效毛细管电泳仪测定小鼠血清和脑组织中铝含量。结果 :血清铝测定的回收率为 96.5%～10 3 % ,脑铝测定的回收率为 92 .2 %～ 10 5.3 % ,有较好的准确性和稳定性。白矾、氢氧化铝和氯化铝各组均出现血清铝含量比对照组显著升高 (P <0 .0 0 1) ,各大剂量组脑铝含量比对照组显著升高 (P <0 .0 0 1) ;停药 2周后 ,除氯化铝大剂量组脑铝含量仍高于对照组外 (P <0 .0 1) ,其余各组均恢复正常。结论 :高效毛细管电泳法可用于测定小鼠血清和脑组织中的铝含量。小鼠口服白矾、氢氧化铝和氯化铝等含铝化合物 60d后 ,均有血、脑铝含量升高 ;停药 14d后血铝可恢复正常 ,氯化铝大剂量组小鼠脑铝恢复较慢。说明铝在小鼠体内的代谢、排泄与所用含铝化合物的化合状态有关。

Objective: To study the effects of alum, a lu minum chloride and aluminum hydroxide on aluminum contents in serum and brain of mice with high performance capillary. Method: 60 days aft er the mice were given daily alum, aluminum chloride and aluminum hydroxide with the same aluminum content of 14.25,57 mg·kg -1 ·d -1 , respectively, the aluminum content in serum and brain of mice were determined with high perfo rmance capillary chromatography. Result: The average rec overies of serum aluminum determination was 96.5%～103%. The average recoveries of brain aluminum assay was 92.2%～105.3%. Except control group, serum al uminum increased obviously. Brain aluminum increased in all the large doses grou ps. 2 weeks after the mice were stopped being giver these drugs, serum and brain aluminum recovered to normal level, except aluminum chloride large doses group. Conclusion : The metabolism and excretion mechanism of aluminum in mice depends on the chemical states of the aluminum compound .