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3号染色体短臂末端两个大片段基因组区域的基因分布与GC含量分析 被引量：2

Comparing Gene Density and GC Content Between Two Large Genomic Blocks at Short Arm of Chromosome 3
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摘要 运用鸟枪法测序技术 ,得到了位于 3p2 5 1和 3p2 6 1区域、大小分别为 32 8kb和 75 3kb的 2个基因组片段 ,分析各片段的GC含量及重复顺序的分布特征 ,并研究不同区域内的基因分布密度。结果表明 :位于 3p2 5 1区域的32 8kb片段具有较高的GC含量 ,在此区域内蛋白编码基因分布密度较高 ,而位于 3p2 6 1区域的 75 3kb片段平均GC含量较低 ,并且是基因贫乏区域。同时发现 :GC rich的SINE类重复顺序在GC含量较高的区域有较高的覆盖率 ,相反AT rich的LINE类重复顺序在GC较低的区域分布较多 。 The present paper is to investigate gene distribution at different genomic region with different local GC content.With shout-gun technology,we sequencing two sequences at 3p25.1 and 3p26.1,at genome size 328 kb and 753 kb respectively.The 328 kb sequence,with an average GC content as high as 47.57%,has high gene density (13.7 gene/100 kb).However,another sequence at 3p26.1 only includes one exon and one intron of large gene GRM7 and the coding sequence size is 11 520 bp only taking a small part(1.31%) of the whole genome region.High percentage distribution of GC-rich repeats of SINEs explain high GC genomic region at 3p25.1 and high concentration of AT-rich repeats of LINEs at 3p26.1 lead to low GC content of the region.Our results suggest isochore structure is the result of coevolution between gene and genome.
作者 牛宇欣 胡松年 杨焕明
机构地区 中国科学院遗传与发育生物学研究所人类基因组中心 北京华大基因研究中心
出处 《Acta Genetica Sinica》 SCIE CAS CSCD 北大核心 2004年第1期13-18,共6页
基金 国家高技术研究发展计划资助项目 (编号 :863 J19) 中国科学院创新工程资助项目 (编号 :KSCX1 D4)~~
关键词 鸟枪法测序 基因密度 GC含量 重复顺序 shotgun sequencing 3p25.1 3p26.1 gene density GC content
分类号 Q987 [生物学—遗传学]
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同被引文献15

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Acta Genetica Sinica
2004年 第1期

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