2N-4N嵌合体小鼠及其在基因功能研究上的应用

Diploid-tetraploid Chimera Mouse and Its Applications in the Study of Gene Function

2N-4N嵌合体是由二倍体(2N)的胚胎细胞(或ES细胞)与四倍体(4N)的胚胎细胞组构成的一种拯救型嵌合体。这种嵌合体由于4N胚胎细胞独特的胚外组织发育特性,从而可获得完全源自2N细胞成份的仔鼠(或胎儿)这一特性最终在ES细胞转基因的基因功能研究中极具重要价值。综述了2N-4N嵌合体研究进展及其在基因功能研究领域的应用。

As a specific type of rescuing chimerism, diploid-tetraploid aggregation approach usually results in pure animals derived predomintly from diploid component, which fulfills its wide application in gene expression and control research. This review hereby summerizes current diploid-tetraploid research progresses and its possible applications in gene function, meanwhile, the relevant prospective has also been envisaged objectively.