细胞培养液乳酸脱氢酶漏出率的比色测定及其应用

Determination and Application of Leakage Rate of Lactate Dehydrogenase in the Cultured Medium of Cells

采用乳酸脱氢酶比色法测定细胞培养液乳酸脱氢酶的漏出率是一个稳定而实用的指标。本法既可客观衡量细胞的受损程度,也可进行药物干预的正确评价,也是进行药物筛选的一种有效方法。

It is a stable and practical criterion to determine the leakage rate of lactate dehydrogenase(LDH) in the cultured medium of cells by the colorimetric method of LDH. The method not only can measure objectively the damaged degree of cells and evaluate properly the role of medicine intervention, but also is a effective way of drug screening.