摘要
目的:探讨细胞因子TGF-β1、IL-4和IFN-γ在肝纤维化形成中变化及意义。方法:采用二硝基亚硝胺(DMN)诱导大鼠肝纤维化模型,细胞生物法测定血清转化生长因子β1(TGF-β1),双抗夹心ELISA法测定血清白细胞介素4(IL-4),肿瘤坏死因子α(TNF-α)和γ-干扰素(IFN-γ),同时进行肝组织学检查和羟脯氨酸(Hyp)测定。结果:TGF-β1、IL-4和IFN-γ在肝纤维化中均是逐步升高,TNF-γ在肝纤维化中前期升高,后期降低;TGF-β1、IL-4和IFN-γ与肝纤维化组织Hyp的相关性较好,r分别为0.71,0.52,和0.62。结论:TGF-β1、IL-4、IFN-γ、TNF-α参与肝纤维化发生的细胞因子网络的调节。
Objective: To study the dynamic change of transform growth factor β1(TCF-β1) .interleukin 4(IL-4), tumor necrosis factor α(TNF-α) and gamma-interferon( IFN-γ) in experimental hepatic fibrosis formation and its significance. Methods: Dimethylnitrosmine (DMN) was used to produce a SD rat model. Serum content of TGF-β1 was examined with cell biology method, and serum content of three another cytokines, including IL-4, TNF-α and IFN-γ with double antibody ELISA method. Liver sections were stained with HE,Masson's trichome and Picric acid-Sirus red stain,respectively. Liver hydroxyproline (Hyp) content was detected. Results: TGF-β1, IL-4 and IFN-γ ascended gradually in the course of the development of hepatic fibrosis. TNF-α ascended in the early stage and descended in the later stage. There was better correlation between the contents of TGF-β1 , IL-4, IFN-γ and Hyp. The coefficient 'r' of these cytokines was 0.71,0.52,and 0.62. Conclusions:The results suggested that the four cytokines had played on important role in the regulation of hepatic fibrosis formation.
出处
《中国民康医学》
2004年第1期1-3,共3页
Medical Journal of Chinese People’s Health
