支架蛋白对丝裂原活化蛋白激酶信号途径中震荡的抑制

SCAFFOLD ELIMINATE THE OSCILLATION IN THE MITOGEN-ACTIVATED PROTEIN KINASE CASCADES

通过一种两体支架蛋白参与的丝裂原活化蛋白激酶 (mitogen activatedproteinkinase,MAPK)信号转导模型 ,研究了支架蛋白参与的存在负反馈的MAPK信号转导途径 .支架蛋白(scaffoldprotein)既可以促进信号过程 ,也可以抑制信号的转导 .由系统中支架蛋白的浓度以及支架与信号分子的相互作用共同决定了增加支架浓度造成的不同结果 .不仅如此 ,系统中支架蛋白浓度的增加 ,还会抑制负反馈系统中出现的持续震荡 .

The mitogen-activated protein kinase (MAPK) cascade is a highly conserved series of three protein kinases implicated in diverse biological process. The scaffold protein is absolutely critical in the signal transduction. It greatly enhances the sensitivity of the cascade to external stimuli. A model involved a two-member scaffold into the protein phosphorylation cascades is developed to study the function of the scaffold in the cascades. Numerical stimulation reveals that the scaffold not only biphasically affects the levels of cascades signaling, but also reduces its threshold properties, the scaffold also affects the input-output function. The scaffold expression will eliminate the sustained oscillations that appear in the system without scaffold.