摘要
根据水牛卵细胞在微管吸吮下的行为，合理地采用内含不可压缩流体固体弹性球壳模型．研究水牛卵细胞透明带的力学性质。通过力学分析，推导出一个近似计算杨氏模量的实验公式。在24-26℃环境温度下，测量了20个培养液中成熟无极体卵细胞的杨氏弹性系数，得到卵细胞透明带的平均弹性系数为2．2×10^4Pa，为今后进行动物卵细胞透明带的力学研究提供了一种有效的测量方法。
In suction experiment of baffalo ovum cultured in vitro, elastic sph er e model with incompressible liquid inside is adopted to study the mechanical pr operties of their zona pellucida. Through mechanical analysis, a formula was de rived to calculate the Young's modulus of zona pellucida from measurements. At temperature of 24~26 ℃, 20 mature but with no polocyte cells were tested. The mean Young's modulus value E is 2.2×10 4 Pa, with the deviation within 30%. This study will provide an effective me thod for gaining the E of zona pellucida for oocytes.
出处
《生物物理学报》
CAS
CSCD
北大核心
2004年第1期73-76,共4页
Acta Biophysica Sinica
基金
国家自然科学基金项目(19902011)
关键词
透明带
微管吸吮
球壳模型
杨氏模量
水牛
卵母细胞
Zona pellucida
Micropipette aspiration
Spherical shell model
Young's modulus
