中国斑茅种质资源分子多态性分析

Molecular polymorphic analyses for the germplasms of Erianthus arundinaceus collected in China

采用ISSR分子标记技术,对采自我国8省区的30个斑茅〔Erianthusarundinaceus(Retz.)Jesw.〕无性系的分子多态性进行了分析,共扩增出87条多态带,平均每个引物扩增的多态带数为5.1条,多态性条带比率(PPB)为64.89%,多态信息量(PIC)为0.58,说明我国斑茅具有较丰富的遗传多样性。根据研究结果进行聚类和主成分分析,可将30个斑茅无性系归为4大类,同一地区的斑茅无性系基本聚在同一类,呈现出一定的地域性分布规律。

Inter-Simple Sequence Repeat (ISSR) molecular markers were used to detect the genetic diversity among 30 clones of Erianthus arundinaceus (Retz.) Jesw. collected from eight provinces and regions in China. Eighty-seven polymorphic bands were produced totally, average 5.1 bands from each pair of ISSR primers, average percentage of polymorphic bands was 64.89%, and mean polymorphism information content was 0.58, which were indicating the rich genetic diversity of E. arundinaceus in China. Based on the cluster and principal component analyses on the genetic characteristics, all the clones could be divided into 4 groups according to the nearest phylogenetic relationship. In most cases, the clones from the same region were in the same group and only the clones from few regions could not be clustered into the same group, which considerably presented the geographical distribution of genetic diversity of the tested clones.