选择性、非选择性NOS抑制剂及L-精氨酸对创伤性休克大鼠的影响

Effect of selective,non-selective nitric oxide synthase inhibitor and L- arginine on traumatic shock in rats

目的 探讨一氧化氮合酶 (NOS)抑制剂及L -精氨酸 (L Arg)对创伤性休克大鼠的治疗作用。方法 建立创伤性休克大鼠模型 ,随机分为对照组、左旋硝基精氨酸甲酯 (L NAME)处理组、氨基胍 (AG )处理组及L -精氨酸 (L Arg)处理组 ,分别于复苏后 1,4,7h检测血清一氧化氮 (NO )浓度 ,复苏后 1h检测骨骼肌、肝脏、小肠的组织氧分压 ,监测血液动力学变化并记录存活时间。结果 与对照组相比 ,L NAME可显著降低血清NO浓度 ,而对组织氧分压及 12 ,2 4h存活率无显著影响 ;AG仅在休克后期使NO浓度明显降低 ,而对早期NO的合成与释放无显著作用 ,AG可提高肝脏、小肠的组织氧分压 ,提高休克模型 12h存活率 ;应用L Arg可显著提高血清NO浓度 ,并可明显改善肝脏、小肠的组织氧分压 ,显著提高 12 ,2 4h存活率。结论 选择性NOS抑制剂AG及L Arg更能改善创伤性休克动物模型的预后。

Objective To investigate the effect of nitric oxide synthase(NOS) inhibitors and L-arginine(L-Arg) on the prognosis of traumatic shock in rats. Methods Traumatic shock models of Sprague-Daulay rats were made and randomly devided into control group (n=24),L-NAME treatment group (n=24),AG treatment group (n=24) and L-Arg treatment group (n=24). Serum nitric oxide(NO) levels and oxygen partial pressure in tissues include skeletal muscles,liver and small intestine were detected at 1h,3h,5h after resuscitation. Meanwhile, hemodynamic data of the rats and their survival rates of 12h and 24h were monitored and recorded. Results Serum NO concentration was statistically lower after resuscitation in L-NAME group than that in control group, while there were no statistical significance of tissues oxygen partial pressure and survival rate in 12、24h between the two groups. AG could decrease serum NO levels only at late stage of traumatic shock,but no effect on the synthesis and relase of NO at early stage of traumati shock.AG could improve tissues oxygen partial pressure of the liver and small intestine, and prolonged the mean survival time. L-Arg could increase serum NO levels, and improve oxygen partial pressure of intestine and significantly increase the survival rate at 12h and 24h in rats with traumatic shock. Conclusions Treatment with AG and L-Arg can improve the prognosis of traumatic shock rats much better than that with L-NAME.