嵌合体在同种心脏移植免疫耐受中的作用

The role of chimerism on immune tolerance to cardiac allografts

目的 探讨嵌合体在同种心脏移植免疫耐受中的作用。 方法 采用大鼠腹部心脏移植模型 ,将 30只L ewis大鼠随机分成正常对照组 ( 组 )、排斥反应组 ( 组 )、免疫耐受组 ( 组 ) ,每组 10只。观察移植心存活时间 ,供心病理学改变 ,供、受者间的混合淋巴细胞反应 (ML R)和脾、胸腺嵌合体。 结果 组供心平均存活时间 (85 .2 8±7.4 8天 )较 组 (7.33± 1.0 3天 )显著长 (P<0 .0 1) ; 组供心见大量炎性细胞浸润 , 组供心仅见少量炎性细胞浸润 ; 组供、受者间 ML R较 组显著低 (P<0 .0 1) ; 组受者的脾、胸腺形成了稳定的供者细胞嵌合体。 结论 移植免疫耐受的受者形成了稳定的中枢和外周嵌合体 ,嵌合体的形成对移植耐受起重要的作用。

Objective To study the role of chimerism on immune tolerance to cardiac allografts. Methods Male DA rat hearts were transplanted to male Lewis rats using Ono's model and randomly divided into three groups: normal control group (group Ⅰ), rejection group (group Ⅱ), immune tolerance group (group Ⅲ). Mean survival time (MST), histological changes, mixed lymphocyte reaction (MLR), chimerism of recipients' spleen and thymus were measured after operation. Results The MST of cardiac allografts in group Ⅲ (85.28±7.48 d) was significantly longer than that in the group Ⅱ (7.33±1.03 d). Only a few inflammatory cells infiltrated in cardiac allografts in group Ⅲ. MLR of group Ⅲ were significantly decreased compared with those of group Ⅰ (P<0.01). Conclusion The chimerism of recipient plays an important role on immune tolerance to cardiac allografts.