PDTC对缺氧/再给氧时血管内皮细胞ICAM-1,VCAM-1表达的作用

The effect of PDTC on ICAM-1,VCAM-1 expression of endothelial cells in hypoxia/reoxygenation-stimulated

目的 为研究心肌缺血 -再灌注损伤的机制和治疗途径 ,检测血管内皮细胞缺氧 /再给氧后细胞间黏附分子 - 1(intracellular adhesion molecule1,ICAM- 1)和血管细胞黏附分子 - 1(vascular cell adhesion molecule1,VCAM-1)的表达 ,探讨抗氧化剂吡咯烷二硫氨基甲酸酯 (pyrrolidine dithiocarbamate,PDTC)对血管内皮细胞表面细胞黏附分子表达的抑制作用。 方法 将培养的人胚肾血管内皮细胞分为 3组 ,缺氧组 :细胞经过缺氧 /再给氧处理 ;PDTC组 :在缺氧前于培养液中加入 PDTC;对照组 :未经处理。以多光子激光共聚焦显微镜分别检测 3组细胞 ICAM- 1、VCAM- 1的表达情况。 结果 对照组内皮细胞表面 ICAM- 1和 VCAM- 1呈较低表达 ,缺氧组呈较高表达 ;PDTC组ICAM- 1和 VCAM- 1的表达明显低于缺氧组 ,但仍高于对照组。 结论 缺氧 /再给氧促进内皮细胞活化 ,增强细胞黏附分子的表达 ,抗氧化剂 PDTC能有效降低 ICAM- 1和 VCAM- 1的表达 ,为心肌缺血 -再灌注损伤的治疗提供理论基础。

Objective To study the mechanisms and treatment of ischemia /reperfusion injury, expression of intracellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) were measured, the effect on suppression of ICAM-1 and VCAM-1 by the pyrrolidine dithiocarbamate (PDTC) were investigated. Methods Endothelial cells were divided into 3 groups, hypoxia group: endothelial cells were exposed in hypoxia condition, then returned to reoxygenation condition; the PDTC group: PDTC was added to the endothelial cells in the culture media before exposing to hypoxia condition; control group: endothelial cells underwent treatment. Confocal microscopy was used to detect expression of ICAM-1 and VCAM-1. Results ICAM-1 and VCAM-1 expression were low in endothelial cells of control group, and increased in hypoxia group . ICAM-1 and VCAM-1 expression of endothelial cells in PDTC group werelower than those in hypoxia group , but higher than those in control group. Conclusions It seems that hypoxia/reoxygenation can activate the endothelial cells and increase the expression of cell adhesion molecules. PDTC can decrease the expression of ICAM-1 and VCAM-1. PDTC may prove benificial in the treatment of ischemia /reperfusion injury.