生长激素对髂内动脉结扎大鼠勃起功能和nNOS神经纤维的影响

Effects of Growth Hormone on Erectile Function and Number of nNOS-containing Nerve Fibers in Internal Iliac Arterial Ligation Rats

目的 :研究生长激素 (GH)对髂内动脉结扎大鼠勃起功能和神经型一氧化氮合酶 (nNOS)神经纤维的影响。方法 :36只成年雄性Wistar大鼠随机均分为 3组 :假手术组、髂内动脉结扎组和GH干预组。于术后 4周末、8周末 ,分别取各组 1/ 2大鼠 ,观察大鼠勃起功能 ,并用免疫组化SP法检测阴茎组织中nNOS神经纤维的数目。 结果 :4周末 ,GH干预组和髂内动脉结扎组在勃起次数和nNOS神经纤维数目上与假手术组相比差异有显著性 (P<0 .0 5 ) ,前两组之间差异无显著性 (P >0 .0 5 ) ,而勃起率 3组之间差异不明显 (P >0 .0 5 )。 8周末 ,3组在勃起次数和勃起率上差异均不明显 ;而阴茎组织中nNOS神经纤维数目 ,GH干预组高于髂内动脉结扎组 (P <0 .0 0 1) ,而与假手术组比较差异无显著性 (P >0 .0 5 )。 结论 :GH可以改善髂内动脉结扎大鼠勃起功能 。

Objective: To investigate the effects of growth hormone (GH) on the erectile function and the number of nNOS-containing nerve fibers in internal iliac arterial ligation rats. Methods: Thirty-six mature male Wistar rats were randomized into 3 groups: GH intervention group, internal iliac arterial ligation group and sham operation group. After four weeks and eight weeks of treatment,one jalf of the rats of each group were selected respectively and tested for erectile function and then sacrificed for the detection of nNOS-containing nerve fibers by streptaridin-peroxidase immunohistochemistry techniques(SP method). Results: After four weeks, the erection frequency and the number of nNOS-containing never fibers in the sham operation group significantly increased compared with the other two groups, which showed no significant difference(P> 0.05). There was no significant difference in erection rate among the three groups. After eight weeks, the number of nNOS-containing never fibers in corpus carvosum in the internal iliac arterial ligation group decreased compared with the other two groups, between which there was no significant difference. And there was no significant difference in erection frequency and erection rate among the three groups. Conclusion: Growth hormone can improve erectile function of internal iliac arterial ligation rats, which can be explained by the increase in the number of nNOS-containing never fibers in corpus carvosum of rats.