前列回春对实验性大鼠前列腺组织血管内皮生长因子表达的影响

Effect of Qianlie Huichun on Prostate Tissue VEGF Expression in Rats

目的 :探计前列回春对实验性大鼠前列腺组织血管内皮生长因子 (VEGF)表达的影响 ,阐明其抗前列腺增生作用。 方法 :雄性SD大鼠 6 0只随机分为正常组 ,模型组 ,雌二醇组 ,前列回春低、中、高剂量组 ,每组 10只。除正常组外 ,其他 5组均去势 ,去势 7d后给大鼠注射丙酸睾酮 4mg·kg-1·d-1,连续 1个月 ;同时给前列回春低、中、高剂量组按 0 .4、0 .8、1.6g·kg-1·d-1的比例灌胃给药 ;雌二醇组皮下注射雌二醇 2 .5mg·kg-1·d-1;模型组和正常组给予等容积蒸馏水。用药 1个月后处死大鼠取前列腺组织 ,用免疫组化方法检测前列腺组织中VEGF阳性细胞表达率。 结果 :前列回春各剂量组与模型组比较差异均有显著性 (P <0 .0 1) ,前列回春中、高剂量组与雌二醇组比较差异也有显著性 (P <0 .0 1)。 结论 :前列回春可抑制实验性大鼠前列腺组织中VEGF的表达 ,其表达量随药量的增加而降低 ,说明该药具有抗前列腺组织新生血管形成。

Objective: To investigate the effect of Qianlie Huichun on the expression of vascular endothelial growth factor(VEGF) in prostate tissues and expound its anti-prostatomegaly action in model rats with prostatic hypertrophy induced by injected testosterone. Methods: A total of 60 male rats were eventy randomized into six groups. All were gelded except the normal control group. After a week, the gelded rats were injected with testosterone(4 mg/kg/d), meanwhile the first group were fed with a small dosage of Qianlie Huichun( 0.4 g/kg/d), the second group with a medium dosage( 0.8 g/kg/d), the third group with a large dosage( 1.6 g/kg/d), and the fourth group injected with estriol( 2.5 mg/kg/d), all for a month. The fifth group were model controls, and the sixth the normal controls, both fed with the same amount of pure water for a month. Then all the six groups of rats were killed and their prostate glands were resected for the examination of the expression rate of VEGF by immunohistochemical method. Results: The difference of VEGF expression between Qianlie Huichun groups and the model group was significant(P< 0.01), and so was it between the medium and large dosages middle, large amount of Qianlie Huichun groups and the estriol group(P< 0.01). Conclusion: Qianlie Huichun depressed the VEGF expression of the prostate gland in model rats, and the expression rate decreased with the increased amount of the drug, which shows that Qianlie Huichun has a definite therapeutic effect on prostatic hypertrophy by depressed the vascular growth of the vessel in the prostate gland.